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Proteus appendicitidis sp. nov., isolated from the appendiceal pus of an appendicitis patient in Yongzhou, China

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Abstract

A Gram-negative, facultatively anaerobic, short rod-shaped bacterium, designated as strain HZ0627T, was isolated from the appendiceal pus of a patient with appendicitis in Yongzhou, Hunan, China. This strain was subjected to comprehensive phenotypic, phylogenetic, and genomic analyses using polyphasic taxonomic methods. Phylogenetic analysis of the 16S rRNA gene sequence revealed that this strain belonged to the genus Proteus and the family Morganellaceae, whereas that based on the rpoB gene sequence and phylogenomic analysis demonstrated that this strain was distinctly separated from other type strains of Proteus species. Moreover, whole-genome-based analyses, including in silico DNA–DNA hybridization (isDDH) and average nucleotide identity (ANI), revealed that strain HZ0627T had much lower isDDH rates (24.5–55.6%) and ANI (82.04–93.90%) than those of the thresholds (i.e., 70% and 95%, respectively) for species delineation, when compared to the type strains of other Proteus species. The cellular fatty acid profile of strain HZ0627T was dominated by C16:0 (34.5%), cyclo C17:0 (25.8%), C14:0 (12.6%), C16:1 iso I/14:0 3-OH (7.7%), C18:1ω7c/18:1ω6c (6.5%), and C16:1ω7c/16:1ω6c (4.9%), which clearly differentiated it from the documented type strains of Proteus species. In addition, several specific physiological traits, including optimal growth temperature, tolerance to sodium chloride, and carbon source utilization, differed from those of other Proteus species. Therefore, we propose the name Proteus appendicitidis sp. nov. for strain HZ0627T (= CCTCC AB 2022380T = KCTC 92986T), which represents the type strain of this novel Proteus species.

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Data availability

The GenBank accession numbers for the 16S rRNA gene and rpoB gene sequences of strain HZ0627T are OQ627395 and OQ630941, respectively. The GenBank accession number for the complete genome sequence of strain HZ0627T is CP127389.

Abbreviations

ANI:

Average nucleotide identity

isDDH:

In silico DNA–DNA hybridization

ML:

Maximum likelihood

LBA:

Luria–bertani agar

CFAs:

Cellular fatty acids

BGCs:

Biosynthesis gene clusters

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Funding

This work was financially supported by the Shenzhen Science and Technology Program (grant number JCYJ20230808105208017) and the National Natural Science Foundation of China [grant number 41907214].

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Authors and Affiliations

  1. Department of Pharmacy, The Central Hospital of Yongzhou, Yongzhou, 425000, Hunan, China

    Jing He & Wangxi Zhou

  2. Department of Clinical Laboratory, The Central Hospital of Yongzhou, Yongzhou, 425000, Hunan, China

    Zhiyun Zhang

  3. Department of Gastrointestinal Surgery, The Central Hospital of Yongzhou, Yongzhou, 425000, Hunan, China

    Haibo Qu, Gongqin Chen & Junfeng Zhou

  4. Department of Gastroenterology and Hepatology, Shenzhen University General Hospital, Shenzhen University, Shenzhen, 518071, Guangdong, China

    Yao Peng & Xunchao Cai

Authors
  1. Jing He
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  2. Zhiyun Zhang
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  3. Haibo Qu
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  4. Gongqin Chen
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  5. Junfeng Zhou
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  6. Wangxi Zhou
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  7. Yao Peng
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  8. Xunchao Cai
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Contributions

J.H. analyzed the data and drafted the manuscript. Z.Z. isolated and purified the bacteria strain. H.Q. and G.C. performed the polyphasic analyses. W.Z., J.Z., and Y.P. reviewed and revised the manuscript. X.C. conceived and funded the study and drafted the manuscript.

Corresponding authors

Correspondence to Yao Peng or Xunchao Cai.

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Conflict of interest

The authors declare that there are no conflicts of interest.

Ethical approval

The study was approved by the Ethics Committee of the Central Hospital of Yongzhou (June 23, 2022; IRB No. 2022062301) and was compliant with all relevant ethical regulations.

Additional information

Communicated by Yusuf Akhter.

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He, J., Zhang, Z., Qu, H. et al. Proteus appendicitidis sp. nov., isolated from the appendiceal pus of an appendicitis patient in Yongzhou, China. Arch Microbiol 206, 171 (2024). https://doi.org/10.1007/s00203-024-03887-8

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  • DOI: https://doi.org/10.1007/s00203-024-03887-8

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