Abstract
A novel strictly anaerobic, Gram-negative bacterium, designated as strain FXJYN30E22T, was isolated from the feces of a healthy woman in Yining county, Xinjiang province, China. This strain was non-spore-forming, bile-resistant, non-motile and rod-shaped. It was found to belong to a single separate group in the Phocaeicola genus based on its 16 S ribosomal RNA (rRNA) gene sequence. Alignments of 16 S rRNA gene sequences showed only a low sequence identity (≤ 95.5 %) between strain FXJYN30E22T and all other Phocaeicola strains in public data bases. The genome (43.0% GC) of strain FXJYN30E22T was sequenced, and used for phylogenetic analysis which showed that strain FXJYN30E22T was most closely related to the type strain Phocaeicola massiliensis JCM 13223T. The average nucleotide identity (ANI) value and digital DNA–DNA hybridization (dDDH) between FXJYN30E22T and P. massiliensis JCM 13223T were 90.4 and 41.9 %, which were lower than the generally accepted species boundaries (94.0 and 70 %, respectively). The major cellular fatty acids and polar lipids were anteiso-branched C15:0 and phosphatidylethanolamine, respectively. The result of genome annotation and KEGG analysis showed that strain FXJYN30E22T contains a number of genes in polysaccharide and fatty acid synthesis that indicated adaptation to the human gut system. Furthermore, a pbpE (penicillin-binding protein) gene was found in the genome of strain FXJYN30E22T but in no other Phocaeicola species, which suggested this gene might be contribute to the adaptive capacity of strain FXJYN30E22T. Based on our data, strain FXJYN30E22T (= CGMCC1.17870T/KCTC25195T) was classified as a novel Phocaeicola species, and the name Phocaeicola faecalis sp. nov., was proposed.
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Data availability and materials
The 16 S rRNA gene sequence of strain FXJYN30E22T is MN854703. The draft genome of FXJYN30E22T has been deposited in the NCBI database (Genome accession number: JADNRM000000000). All data generated or analysed during this study are included in this published article and its supplementary information files.
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Funding
This work was supported by the Natural Science Foundation of Jiangsu Province [BK20200084];The National Natural Science Foundation of China [No. 31820103010]; The key soft science research project of Wuxi Science and Technology Association [KX-20-A26]. The National first-class discipline program of Food Science and Technology [JUFSTR20180102]; and Collaborative innovation center of food safety and quality control in Jiangsu Province.
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CW, ZZ, QZ and WC designed the study; CW, SL, ZY, LY and FT performed research and analyzed data. CW wrote the manuscript. All of the authors contributed to the manuscript revision process and read and approved the submitted version.
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The human participants in this study were approved by the Ethics Committee in Jiangnan University, China. The fecal sample from healthy person was for public health purposes and these were the only human materials used in present study. Written informed consent for the use of their fecal samples were obtained from the participants or their legal guardians. No human experiments were involved. The collection of fecal sample had no risk of predictable harm or discomfort to the participants.
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Chen Wang and Sijia Li have contributed equally to this work.
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Wang, C., Li, S., Zhang, Z. et al. Phocaeicola faecalis sp. nov., a strictly anaerobic bacterial strain adapted to the human gut ecosystem. Antonie van Leeuwenhoek 114, 1225–1235 (2021). https://doi.org/10.1007/s10482-021-01595-7
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DOI: https://doi.org/10.1007/s10482-021-01595-7