Abstract
A Gram-stain-positive, anaerobic, motile, and short rod-shaped bacterium, designated KGMB12511T, was isolated from the feces of healthy Koreansubjects. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain KGMB12511T was closely related to Gordonibacter pamelaeae 7-10-1-bT (95.2%). The draft genome of KGMB12511T comprised 33 contigs and 2,744 protein-coding genes. The DNA G + C content was 59.9% based on whole-genome sequences. The major cellular fatty acids (>10%) of strain KGMB12511T were C18:1 cis9, C18:1 cis9 DMA (dimethylacetal), and C16:0 DMA. The predominant polar lipids included a diphosphatydilglycerol, four glycolipids, and an unidentified phospholipid. The major respiratory quinones were menaquinone 6 (MK-6) and monomethylmenaquinone 6 (MMK-6). Furthermore, HPLC analysis demonstrated the ability of strain KGMB12511T to convert ellagic acid into urolithin. Based on a comprehensive analysis of phenotypic, chemotaxonomic, and phylogenetic data, strain KGMB12511T represents a novel species in the genus Gordonibacter. The type strain is KGMB12511T (= KCTC 25343T = NBRC 116190T).
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Data availability
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and the draft genome sequence of type strain KGMB12511T are OQ998951 and JASJEU000000000, respectively.
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Acknowledgements
We thank Professor Bernhard Schink and Aharon Oren for his advice in naming the novel strain.
Funding
This work was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2016M3A9F3947962) and Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (KGM5232322).
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Conceptualization: J-SL. Formal analysis and investigation: HSK and J-SK. Writing—original draft preparation: HSK. Writing—review and editing: J-SK and J-SL. Resources: HSK, J-SK, MKS, MKE, HED, JHL, S-HP, SWK, DHL, HY, and JHL. Supervision and funding acquisition: J-SL. All authors read and approved the final manuscript.
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This study was conducted in accordance with the IRB regulation at KCTC (P01-201702-31-007).
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Kim, H.S., Kim, JS., Suh, M.K. et al. Gordonibacter faecis sp. nov., producing urolithin C from ellagic acid, isolated from feces of healthy Korean subjects. Arch Microbiol 206, 108 (2024). https://doi.org/10.1007/s00203-024-03844-5
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DOI: https://doi.org/10.1007/s00203-024-03844-5