Abstract
Two obligately anaerobic, Gram-stain-negative, non-motile, non-spore-forming and short rod shaped bacteria, designated KGMB07931T and KGMB10229, were isolated from faeces of two Korean persons. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains KGMB07931T and KGMB10229 were very similar to each other (99.9%) and grouped within the genus Bacteroides, displaying the highest similarity with Bacteroides uniformis ATCC 8492T (97.5%), Bacteroides rodentium JCM 16496T (96.6%), and Bacteroides fluxus YIT 12057T (94.5%). Both strains grew optimally at 37 °C and pH 7.5 in the presence of 0.5% (w/v) NaCl. The complete genome of KGMB07931T comprises 3,335 protein-coding genes with a total of 4,240,638 bp and an average G + C content of 46.3 mol%. The major fatty acids were C18:1 cis9, anteiso-C15:0, iso-C15:0, and Summed Feature 11 (iso-C17:0 3OH and/or C18:2 DMA); the predominant respiratory quinones were MK-9 and MK-10; the major fermentation end products were acetate and isobutyrate. The genome of strain KGMB07931T encoded the starch utilization system (Sus) operon, susABCDEFG, suggesting that this strain uses many complex polysaccharides that cannot be digested by humans. Based on polyphasic taxonomic data, strains KGMB07931T and KGMB10229 represent a novel species within the genus Bacteroides, for which the name Bacteroides humanifaecis sp. nov. is proposed. The type strain is KGMB07931T (= KCTC 25160T = NBRC 115005T).
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Acknowledgements
We thank Professor Bernhard Schink and Aharon Oren for his advice in naming the novel strain.
Funding
This work was supported by the Bio & Medical Technology Development program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science (NRF-2016M3A9F3947962) and ICT (MSIT) of the Republic of Korea and a grant from the Korea Research Institute of Bioscience& Biotechnology (KRIBB) Research initiative program.
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All authors participating in this study were supported by NRF-2016M3A9F3947962 [Supervision: JSL]. Sampling was performed by DHL and HY in accordance with the IRB regulation. Isolation of gut bacteria and taxonomy analysis were performed by JSK, MKS, MKE, JHL, SHP, and SWK. Whole-genome analysis was performed by JHL. The first draft of the manuscript was written by HSK and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript. Conceptualization: JSL. Formal analysis and investigation: HSK. Writing—original draft preparation: HSK. Writing—review and editing: JSK and JSL. Funding acquisition: JSL. Resources: JSK, MKS, MKE, JHL, SHP, SWK, DHL, HY, and JHL. Supervision: JSL.
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This study was conducted in accordance with the IRB regulation at KCTC (P01-201702–31-007).
Nucleotide sequence accession number
The 16S rRNA gene sequences of strains KGMB07931T and KGMB10229 has been deposited in GenBank under MW228414 and MZ497325, respectively. The GenBank/EMBL/DDBJ accession numbers for the complete genome sequences of strains KGMB07931T and KGMB10229 are CP084680 and CP085132, respectively.
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Communicated by Erko Stackebrandt.
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Kim, H.S., Kim, JS., Suh, M.K. et al. Bacteroides humanifaecis sp. nov., isolated from faeces of healthy Korean. Arch Microbiol 204, 357 (2022). https://doi.org/10.1007/s00203-022-02967-x
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DOI: https://doi.org/10.1007/s00203-022-02967-x