Abstract
Enterococcus mundtii AM_AQ_BC8 isolated from biofouled filtration membrane was characterised as a potential probiotic bacterium showing strong L-lactic acid-producing capability. Experimental studies revealed that E. mundtii AM_AQ_BC8 possess antibiofilm and antimicrobial ability too, as tested against strong biofilm-forming bacteria like Pseudomonas spp. The present study has evaluated the genetic potential of E. mundtii AM_AQ_BC8 through genome sequencing. Whole genome analysis revealed the presence of key genes like ldh_1 and ldh_2 responsible for lactic acid production along with genes encoding probiotic features such as acid and bile salt resistance (dnaK, dnaJ, argS), fatty acid synthesis (fabD, fabE) and lactose utilisation (lacG, lacD). The phylogenomic analysis based on OrthoANI (99.85%) and dDDH (96.8%) values revealed that the strain AM_AQ_BC8 shared the highest homology with E. mundtii. The genome sequence of strain AM_AQ_BC8 has been deposited to NCBI and released with GenBank accession no. SAMN32531201. The study primarily demonstrated the probiotic potential of E. mundtii AM_AQ_BC8 isolate, for L-lactate synthesis in high concentration (8.98 g/L/day), which also showed anti-biofilm and antimicrobial activities.
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Data availability
The whole genome sequence of E. mundtii AM_AQ_BC8 was deposited to NCBI GenBank under the BioProject number (PRJNA917242) and the accession number (SAMN32531201).
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The authors acknowledge Director, CSIR-NEERI and AcSIR-NEERI for providing support and essential resources for research work. The manuscript has been internally checked for plagiarism through iThenticate software [KRC No: CSIR-NEERI/KRC/2023/SEP/EBGD/3]. Anuja Maitreya is thankful to the Council for Scientific and Industrial Research (CSIR), New Delhi for Senior Research Fellowship (SRF) [31/016(0137)/2020-EMR-I] for carrying out this research. Also, the authors acknowledge the Indo-Egypt Project (DST/INT/Egypt/P-03/2019) Department of Science and Technology, International Bilateral Cooperation Division, New Delhi.
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The authors are grateful to CSIR-NEERI for providing infrastructure facilities, AcSIR- NEERI and the Council of Scientific and Industrial Research (CSIR) Fellowship of Anuja Maitreya, New Delhi 20 [31/016(0137)/2020-EMR-I] for financial support. Also, funds from the Indo-Egypt Project (DST/INT/Egypt/P-03/2019) Department of Science and Technology, International Bilateral Cooperation Division, New Delhi are acknowledged.
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Maitreya, A., Qureshi, A. Genomic and phenotypic characterisation of Enterococcus mundtii AM_AQ_BC8 for its anti-biofilm, antimicrobial and probiotic potential. Arch Microbiol 206, 84 (2024). https://doi.org/10.1007/s00203-023-03816-1
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DOI: https://doi.org/10.1007/s00203-023-03816-1