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Microbacterium aquilitoris sp. nov. and Microbacterium gwkjiense sp. nov., isolated from beach

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Abstract

Three Gram-reaction-positive bacterial strains, designated KSW-18T, KSW2-22, and KSW4-11T, were isolated from seawater, and two dried seaweed samples collected at Gwakji Beach in Jeju, Republic of Korea, respectively, and their taxonomic positions were examined by a polyphasic approach. The 16S rRNA gene phylogeny showed that strain KSW4-11T was tightly associated with Microbacterium oleivorans NBRC 103075T, while strains KSW-18T and KSW2-22 formed a distinctive subline at the base of a clade including the above two strains. The three isolates showed high sequence similarity with one another (99.7–99.9%; 1–4 nt differences) and Microbacterium oleivorans (99.8–99.9%; 1–3 nt differences). The chemotaxonomic features were typical for the genus Microbacterium; Lysine as the diagnostic diamino acid and N-glycolylated muramic acid of the peptidoglycans, the predominant menaquinones of MK-11, MK-10 and MK-12, the major fatty acids of anteiso-C15:0 and anteiso-C17:0, and the major polar lipids including diphosphatidylglycerol, phosphatidylglycerol, and two or three unidentified glycolipids. In core genome-based phylogenetic tree, strains KSW-18T and KSW2-22 were closely associated with Microbacterium oleivorans NBRC 103075T, while strain KSW4-11T formed a distinctive subline at the base of a clade including the above three strains, in contrast to the 16S rRNA gene tree. Strains KSW-18T and KSW2-22 shared an OrthoANIu of 98.6% and a digital DNA–DNA hybridization of 87.6% with each other, representing that they were strains of a species, while the OrthoANIu and digital DNA–DNA hybridization values between strains KSW-18T and KSW4-11T, and between both of these isolates and all members of the genus Microbacterium were ≤86.5% and ≤30.7%, respectively. The analyses of overall genomic relatedness indices and phenotypic distinctness support that the three isolates represent two new species of the genus Microbacterium. Based on the results obtained here, Microbacterium aquilitoris sp. nov. (type strain KSW-18T = KCTC 49623T = NBRC 115222T) and Microbacterium gwkjiense sp. nov. (type strain KSW4-11T = KACC 23321T = DSM 116380T) are proposed.

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Data availability

The DDBJ/ENA/GenBank accession numbers for 16S rRNA gene and genome sequences are OR039658 and JAUZVV010000000, respectively.

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Acknowledgements

The authors are grateful to Professor Aharon Oren (The Hebrew University of Jerusalem, Israel) for help in preparing species names.

Funding

This work was performed by the financial support of BioPS Co., Ltd.

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Author notes

  1. Soon Dong Lee and In Seop Kim have contributed equally to this work.

Authors and Affiliations

  1. Institute of Jeju Microbial Resources, BioPS Co., Ltd, Jeju, 63243, Republic of Korea

    Soon Dong Lee & Hong Lim Yang

  2. Korean Collection for Type Cultures, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea

    Ji-Sun Kim

  3. Department of Biological Sciences and Biotechnology, Hannam University, Daejeon, 34054, Republic of Korea

    In Seop Kim

  4. BioPS Co., Ltd, Daejeon, 34054, Republic of Korea

    In Seop Kim

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  1. Soon Dong Lee
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  2. Hong Lim Yang
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Contributions

SDL performed bacterial isolation and bioinformatic analyses. HLY carried out phenotypic assays and data collection. JSK performed the preparation and analysis of fatty acids. SDL wrote the manuscript. ISK made financial support. All authors read and approved the final manuscript.

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Correspondence to Soon Dong Lee.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Communicated by Yusuf Akhter.

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The DDBJ/ENA/GenBank accession numbers for 16S rRNA gene sequences of strains KSW-18T, KSW2-22 and KSW4-11T are OR039656-OR039658, respectively. The DDBJ/ENA/GenBank accession numbers of genome sequences of strains KSW-18T, KSW2-22 and KSW4-11T are JAUZVT010000000-JAUZVV010000000, respectively.

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Lee, S.D., Yang, H.L., Kim, JS. et al. Microbacterium aquilitoris sp. nov. and Microbacterium gwkjiense sp. nov., isolated from beach. Arch Microbiol 206, 100 (2024). https://doi.org/10.1007/s00203-023-03804-5

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  • DOI: https://doi.org/10.1007/s00203-023-03804-5

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