Abstract
A novel decalin derivative, trans-1-oxo-2,4-diacetylaminodecalin (1) with anti-Candida activity, had been isolated from Streptomyces chrestomyceticus strain ADP4. The structure of the compound was determined from the analysis of spectral data (LCMS/MS, UV, FTIR, 1D- and 2D-NMR). The anti-Candida activity of 1 was specific to Candida albicans and Candida auris. Further, it displayed inhibition of the early-stage biofilm of C. albicans. In-silico analysis of the compound revealed its drug likeness properties without any violations and PAINS alert when investigated for ADME properties. Along with the overall bioavailability, compound 1 did not show any predicted bioaccumulation and mutagenicity in the analysis by TEST software. Non-cytotoxic property was further confirmed by in-vitro assay on the HepG2 cell line.
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Acknowledgements
The work has been supported by Science and Engineering Research Board, Department of Science and Technology, Government of India through the Research Grant: SERB-EMR/2017/000254 to AKD.
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This study was funded by Science and Engineering Research Board, Department of Science and Technology, Government of India, New Delhi
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AKD conceptualized this study and developed the research plan. RS performed experimental work and analyzed the data. MA elucidated structure of the compound from analysis of spectral data. AKD organized grant, resources and supervised the work. RS wrote the manuscript, which was reviewed and finalized by AKD. Final version of the manuscript was approved by all the authors.
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Singh, R., Ali, M. & Dubey, A.K. Identification and characterization of a novel decalin derivative with anti-Candida activity from Streptomyces chrestomyceticus strain ADP4. Arch Microbiol 206, 50 (2024). https://doi.org/10.1007/s00203-023-03788-2
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DOI: https://doi.org/10.1007/s00203-023-03788-2