Abstract
Obesity is a global health threat that causes various complications such as type 2 diabetes and nonalcoholic fatty liver disease. Gut microbiota is closely related to obesity. In particular, a higher Firmicutes to Bacteroidetes ratio has been reported as a biomarker of obesity, suggesting that the phylum Bacteroidetes may play a role in inhibiting obesity. Indeed, the genus Bacteroides was enriched in the healthy subjects based on metagenome analysis. In this study, we determined the effects of Bacteroides stercoris KGMB02265, a species belonging to the phylum Bacteroidetes, on obesity both in vitro and in vivo. The cell-free supernatant of B. stercoris KGMB02265 inhibited lipid accumulation in 3T3-L1 preadipocytes, in which the expression of adipogenic marker genes was repressed. In vivo study showed that the oral administration of B. stercoris KGMB02265 substantially reduced body weight and fat weight in high-fat diet induced obesity in mice. Furthermore, obese mice orally administered with B. stercoris KGMB02265 restored glucose sensitivity and reduced leptin and triglyceride levels. Taken together, our study reveals that B. stercoris KGMB02265 has anti-obesity activity and suggests that it may be a promising candidate for treating obesity.
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Funding
This work was supported by the Bio & Medical Technology Development program (NRF-2016M3A9F3947962 and NRF-2019M3A9F3065226) of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT) of the Republic of Korea, and a grant from the Korea Research Institute of Bioscience & Biotechnology (KRIBB) Research initiative program.
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SWR performed all the experiments and data analysis, and wrote the manuscript. JCM, BSO, SYY, JEB and ESH helped the experimental design and data interpretation. J-HJ and JHL edited the manuscript and supervised this study. All authors approved the manuscript.
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Communicated by Yusuf Akhter.
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Ryu, S.W., Moon, J.C., Oh, B.S. et al. Anti-obesity activity of human gut microbiota Bacteroides stercoris KGMB02265. Arch Microbiol 206, 19 (2024). https://doi.org/10.1007/s00203-023-03750-2
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DOI: https://doi.org/10.1007/s00203-023-03750-2