Abstract
Microbial biodegradation serves as an effective approach to treat oil pollution. However, the application of such methods for the degrading long-chain alkanes still encounters significant challenges. Comparative proteomics has extensively studied the intracellular proteins of bacteria that degrade short- and medium-chain alkanes, but the role and mechanism of extracellular proteins in many microorganism remain unclear. To enhance our understanding of the roles of extracellular proteins in the adaptation to long-chain alkanes, a label-free LC–MS/MS strategy was applied for the relative quantification of extracellular proteins of Pseudomonas aeruginosa SJTD-1-M (ProteomeXchange identifier PXD014638). 444 alkane-sentitive proteins were acquired and their cell localization analysis was performed using the Pseudomonas Genome Database. Among them, 111 proteins were found to be located in extracellular or Outer Membrane Vesicles (OMVs). The alkane-induced abundance of 11 extracellular or OMV target proteins was confirmed by parallel reaction monitoring (PRM). Furthermore, we observed that the expression levels of three proteins (Pra, PA2815, and FliC) were associated with the carbon chain length of the added alkane in the culture medium. The roles of these proteins in cell mobility, alkane emulsification, assimilation, and degradation were further discussed. OMVs were found to contain a number of enzymes involved in alkane metabolism, fatty acid beta-oxidation, and the TCA cycle, suggesting their potential as sites for facilitated alkane degradation. In this sense, this exoproteome analysis contributes to a better understanding of the role of extracellular proteins in the hydrocarbon treatment process.
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Data availability
The data that support the findings of this study are openly available in the Proteome Xchange depository at http://www.ebi.ac.uk/pride/archive/projects/PXD014638, reference number PXD014638.
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We are grateful to Prof. Rubing Liang and Jianhua Liu of the School of Life Science and Biotechnology, Shanghai Jiao Tong University for the P. aeruginosa SJTD-1-M stain.
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All authors contributed to the conception and design of the study. The study was designed by JLH and XPL. Material preparation was performed by YD and XFZ. Data collection was performed by YD and JLH. Data analysis was carried out by YD and XFZ. The first draft of the manuscript was written by YD and JLH. All authors commented on earlier versions of the manuscript. All authors read and approved the final version of the manuscript.
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Dou, Y., Zhou, X., Liu, X. et al. Exoproteome analysis of Pseudomonas aeruginosa response to high alkane stress. Arch Microbiol 206, 51 (2024). https://doi.org/10.1007/s00203-023-03749-9
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DOI: https://doi.org/10.1007/s00203-023-03749-9