Abstract
Pollution due to textile dye effluent mishandling is hazardous to ecosystems and to the living beings inhabiting them. This can cause retarded photosynthesis, disrupted fish day/night cycles, unbalanced bacterial populations, and decreased oxygen concentration in contaminated water, leading to low habitability. In this study, we aimed to isolate and characterize the microorganisms found in Indonesian cassava-based fermented food tapai starter cultures as a source of potential microbes for the biological remediation of textile dye pollutants. Microorganisms in the tapai starter culture were screened for their decolorization activity via spread-culture inoculation on a solid growth medium supplemented with textile dyes. Isolated microorganisms were selected based on their ability to secrete textile dye-decolorizing extracellular enzymes via increased light penetration after incubation of the cell-free supernatant (CFS) containing extracellular enzymes in textile dye solutions. Isolate JSP1 was the only bacterium capable of producing malachite green (MG)-decolorizing extracellular enzymes, which enabled it to survive and decolorize MG up to 375 ppm. Moreover, isolate JSP1 CFS was able to optimally decolorize 75% of MG at 100 ppm, but its activity was diminished at concentrations > 350 ppm. Colony and cellular morphology, biochemistry, and 16S rRNA tests revealed that the isolate was of Ralstonia mannitolilytica. Therefore, R. mannitolilytica isolate JSP1 may be a potential bioremediation agent for MG.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
References
Abbaszade G, Szabó A, Vajna B, Farkas R, Szabó C, Tóth E (2020) Whole genome sequence analysis of Cupriavidus campinensis S14E4C, a heavy metal resistant bacterium. Mol Biol Rep 47:3973–3985. https://doi.org/10.1007/s11033-020-05490-8
Alaya V, Kodi RK, Ninganna E, Gowda B, Shivanna MB (2021) Decolorization of Malachite green dye by Stenotrophomonas maltophilia a compost bacterium. Bull Natl Res Cent 45:1–13. https://doi.org/10.1186/s42269-021-00518-w
Al-Tohamy R, Ali SS, Li F, Okasha KM, Mahmoud YAG, Elsamahy T, Jiao H, Fu Y, Sun J (2022) A critical review on the treatment of dye-containing wastewater: ecotoxicological and health concerns of textile dyes and possible remediation approaches for environmental safety. Ecotoxicol Environ Saf 231:1–17. https://doi.org/10.1016/j.ecoenv.2021.113160
Berradi M, Hsissou R, Khudhair M, Assouag M, Cherkaoui O, El Bachiri A, El Harfi A (2019) Textile finishing dyes and their impact on aquatic environs. Heliyon 5:1–11. https://doi.org/10.1016/j.heliyon.2019.e02711
Bilal M, Rasheed T, Sosa-Hernández JE, Raza A, Nabeel F, Iqbal HMN (2018) Biosorption: an interplay between marine algae and potentially toxic elements—a review. Mar Drugs 16:1–16. https://doi.org/10.3390/md16020065
Deshkar S, Patil N, Sawant P (2022) Ralstonia mannitolilytica bacteremia in an immunocompromised patient: case report and review. Int J Curr Sci Res Rev 5:418–423. https://doi.org/10.47191/ijcsrr/V5-i2-15
Gan L, Zhou F, Owens G, Chen Z (2018) Burkholderia cepacia immobilized on eucalyptus leaves used to simultaneously remove malachite green (MG) and Cr(VI). Colloids Surf B Biointerfaces 172:526–531. https://doi.org/10.1016/j.colsurfb.2018.09.008
Guerrero R (2001) Bergey’s manuals and the classification of prokaryotes. Int Microbiol 4:103–109
Huang H, Zhao Y, Xu Z, Ding Y, Zhang W, Wu L (2018) Biosorption characteristics of a highly Mn(II)-resistant Ralstonia pickettii strain isolated from Mn ore. PLoS ONE 13:1–17. https://doi.org/10.1371/journal.pone.0203285
Huang N, Mao J, Zhao Y, Hu M, Wang X (2019) Multiple transcriptional mechanisms collectively mediate copper resistance in Cupriavidus gilardii CR3. Environ Sci Technol 53:4609–4618. https://doi.org/10.1021/acs.est.8b06787
Irawati W, Winoto SE, Kusumawati L, Pinontoan R (2021) Indigenous multiresistant bacteria of Cupriavidus pauculus IrC4 isolated from Indonesia as a heavy metal bioremediation agent. Biodiversitas 22:3349–3355. https://doi.org/10.13057/biodiv/d220641
Kabeer FA, John N, Abdulla MH (2019) Biodegradation of malachite green by a newly isolated Bacillus vietnamensis sp. MSB17 from continental slope of the Eastern Arabian Sea: enzyme analysis, degradation pathway and toxicity studies. Biorem J 23:334–342. https://doi.org/10.1080/10889868.2019.1671790
Kanti A, Sukara E, Sukiman H, Lisdiyanti P, Melliawati R, Ratnakomala S, Lekatompessy SJR, Anindyawati T, Widyastuti Y, Yopi (2020) Exploring Indonesian microbial genetic resources for industrial application. LIPI Press, Indonesia, pp 1–14
Kweon OJ, Lim YK, Kim HR, Kim T, Ha S, Lee M (2020) Isolation of a novel species in the genus Cupriavidus from a patient with sepsis using whole genome sequencing. PLoS ONE 15:1–11. https://doi.org/10.1371/journal.pone.0232850
Lellis B, Fávaro-Polonio CZ, Pamphile JA, Polonio JC (2019) Effects of textile dyes on health and the environment and bioremediation potential of living organisms. Biotechnol Res Innov 50:1–16. https://doi.org/10.1016/j.biori.2019.09.001
Li S, Yu Y, Chen J, Guo B, Yang L, Ding W (2016) evaluation of the antibacterial effects and mechanism of action of protocatechualdehyde against Ralstonia solanacearum. Mol 21:754. https://doi.org/10.3390/molecules21060754
Li X, Yan Z, Gu D, Li D, Tao Y, Zhang D, Su L, Ao Y (2019) Characterization of cadmium-resistant rhizobacteria and their promotion effects on Brassica napus growth and cadmium uptake. J Basic Microbiol 59:579–590. https://doi.org/10.1002/jobm.201800656
Li X, Chen Y, Du X, Zheng J, Lu D, Liu Z (2021a) Ecological response in the integrated process of biostimulation and bioaugmentation of diesel-contaminated. Soil Appl Sci 11:1–19. https://doi.org/10.3390/app11146305
Li X, Wu S, Dong Y, Fan H, Bai Z, Zhuang X (2021b) Engineering microbial consortia towards bioremediation. Water 13:1–10. https://doi.org/10.3390/w13202928
Liu Q (2020) Pollution and treatment of dye waste–water. IOP Conf Ser: Earth Environ Sci 514:1–8. https://doi.org/10.1088/1755-1315/514/5/052001
Liu S, Xu X, Kang Y, Xiao Y, Liu H (2020) Degradation and detoxification of azo dyes with recombinant ligninolytic enzymes from Aspergillus sp. with secretory overexpression in Pichia pastoris. R Soc Open Sci 7:1–15. https://doi.org/10.1098/rsos.200688
Macagnan KL, Alves MI, Rodrigues AÁ, Furlan L, da Silva RR, Diaz de Oliveira P, Vendruscolo CT, da Silveira MA (2017) Complete factorial design to adjust pH and sugar concentrations in the inoculum phase of Ralstonia solanacearum to optimize P(3HB) production. PLoS ONE 12:e0180563. https://doi.org/10.1371/journal.pone.0180563
Markandeyaa MD, Shukla SP (2022) Hazardous consequences of textile mill effluents on soil and their remediation approaches. Chem Eng Technol 7:1–8. https://doi.org/10.1016/j.clet.2022.100434
Medunić G, Singh PK, Singh AL, Rai A, Rai S, Jaiswal MK, Obrenović Z, Petković Z, Janeš M (2019) Use of bacteria and synthetic zeolites in remediation of soil and water polluted with superhigh-organic-sulfur Raša Coal (Raša Bay, North Adriatic, Croatia). Water 11:1419–1432. https://doi.org/10.3390/w11071419
Michelle, Siregar RN, Sanjaya A, Lucy J, Pinontoan R (2020) Methylene blue decolorizing bacteria isolated from water sewage in Yogyakarta, Indonesia. Biodiversitas 21:1136–1141. https://doi.org/10.13057/biodiv/d210338
Moreno ZR, Herrera JS (2022) Biodegradation of Erionyl Turquoise azoic textile dye from Chlorella vulgaris microalgae in simulated wastewater. J Text Eng Fash Technol 8:121–124. https://doi.org/10.15406/jteft.2022.08.00309
Nabilah B, Purnomo AS, Rizqi HD, Putro HS, Nawfa R (2022) The effect of Ralstonia pickettii bacterium addition on methylene blue dye biodecolorization by brown-rot fungus Daedalea dickinsii. Heliyon 8:1–7. https://doi.org/10.1016/2Fj.heliyon.2022.e08963
Papagrigorakis E, Vavourakis M, Vlachos C, Zachariou D, Galanis A, Marougklianis V, Polyzois V, Pneumaticos S (2022) Osteomyelitis caused by Ralstonia mannitolilytica, a rare opportunistic pathogen. Cureus 14:e24151. https://doi.org/10.7759/cureus.24151
Paudel S, Dobhal S, Alvarez AM, Arif M (2020) Taxonomy and phylogenetic research on Ralstonia solanacearum species complex: a complex pathogen with extraordinary economic consequences. Pathogens 9:886–901. https://doi.org/10.3390/pathogens9110886
Pinontoan R, Supandi T, Andhika J, Sutanto MI, Victor H (2019) Removal of malachite green toxicity using water hyacinth Eichhornia crassipes biomass. IOP Conf Ser: Earth Environ Sci 346:1–9. https://doi.org/10.1088/1755-1315/346/1/012006
Purnomo AS, Maulianawati D, Kamei I (2019) Ralstonia pickettii enhance the DDT biodegradation by Pleurotus eryngii. J Microbiol Biotechnol 29:1424–1433. https://doi.org/10.4014/jmb.1906.06030
Rahman MM, Mollah MYA, Rahman MM, Susan MABH (2013) Electrochemical behavior of malachite green in aqueous solutions of ionic surfactants. ISRN Electrochem 2013:1–10. https://doi.org/10.1155/2013/839498
Rajendran UD, Sundaramoorthy S, Sethuraman G (2021) Ralstonia mannitolilytica sepsis in neonatal intensive care unit – Be(a)ware of the multidrug resistant nosocomial bug. SAGE 52:216–217. https://doi.org/10.1177/00494755211036557
Rawat D, Sharma U, Poria P, Finlan A, Parker B, Sharma RS, Mishra V (2022) Iron-dependent mutualism between Chlorella sorokiniana and Ralstonia pickettii forms the basis for a sustainable bioremediation system. ISME Commun 2:1–14. https://doi.org/10.1038/s43705-022-00161-0
Rizk N, Eldourghamy A, Aly SA, Sabae SZ, Hassan AS (2020) Production of lignin peroxidase from aquatic bacteria, Alcaligenes aquatilis. Egypt J Aquat Biol Fish 24:213–223. https://doi.org/10.21608/ejabf.2020.90301
Said M, van Hougenhouck-Tulleken W, Naidoo R, Mbelle N, Ismail F (2020) Outbreak of Ralstonia mannitolilytica bacteraemia in patients undergoing haemodialysis at a tertiary hospital in Pretoria, South Africa. Antimicrob Resist Infect Control 9:117–123. https://doi.org/10.1186/s13756-020-00778-7
Sayed K, Baloo L, Sharma NK (2021) Bioremediation of total petroleum hydrocarbons (TPH) by bioaugmentation and biostimulation in water with floating oil spill containment booms as bioreactor basin. Int J Environ Res Public Health 18:1–26. https://doi.org/10.3390/ijerph18052226
Shah MP, Rodriguez-Couto S, Kapoor RT (2022) Development in wastewater treatment research and processes. Elsevier, The Netherlands, pp 175–193
Shankar M, Rampure S, Siddini V, Ballal HS (2018) Outbreak of Ralstonia mannitolilytica in hemodialysis unit: a case series. Indian J Nephrol 28:323–326. https://doi.org/10.4103/ijn.IJN_77_17
Siddiqui T, Patel SS, Sinha R, Ghoshal U, Sahucorresponding C (2022) Ralstonia mannitolilytica: an emerging multidrug-resistant opportunistic pathogen in a tertiary care hospital setting. Access Microbiol 4:acmi000367. https://doi.org/10.1099/acmi.0.000367
Slama HB, Bouket AC, Pourhassan Z, Alenezi FN, Silini A, Cherif-Silini H, Oszako T, Luptakova L, Golinska P, Belbahri L (2021) Diversity of synthetic dyes from textile industries, discharge impacts and treatment methods. Appl Sci 11:1–21. https://doi.org/10.3390/app11146255
Song J, Han G, Wang Y, Jiang X, Zhao D, Li M, Yang Z, Ma Q, Parales RE, Ruan Z, Mu Y (2020) Pathway and kinetics of malachite green biodegradation by Pseudomonas veronii. Sci Rep 10:1–11. https://doi.org/10.1038/s41598-020-61442-z
Sosa-Martínez JD, Balagurusamy N, Montañez J, Peralta RA, de Fátima P-M-M, Bracht A, Peralta RM, Morales-Oyervides L (2020) Synthetic dyes biodegradation by fungal ligninolytic enzymes: process optimization, metabolites evaluation and toxicity assessment. J Hazard Mater 400:1–14. https://doi.org/10.1016/j.jhazmat.2020.123254
Spiekermann P, Rehm BHA, Kalscheuer R, Baumeister D, Steinbüchel A (1999) A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. Arch Microbiol 171:73–80. https://doi.org/10.1007/s002030050681
Tamura K, Stecher G, Kumar S (2021) MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38:3022–3027
Thoa LTK, Thao TTP, Hung NB, Khoo KS, Quang HT, Lan TT, Hoang VD, Park SM, Ooi CW, Show PL, Huy ND (2022) Biodegradation and detoxification of malachite green dye by extracellular laccase expressed from Fusarium oxysporum. Waste Biomass Valoriz 13:2511–2518. https://doi.org/10.1007/s12649-022-01692-2
Thuraisami S, Lakshmi SL (2021) Degradation of food colours using probiotic bacteria Lactobacillus Acidophilus and Lactobacillus Fermentum. Int J Sci Res Growth 6211:1570–1574
Vaneechoutte M, De Baere T, Wauters G, Steyaert S, Claeys G, Vogelaers D, Verschraegen G (2001) One case each of recurrent meningitis and hemoperitoneum infection with Ralstonia mannitolilytica. J Clin Microbiol 39:4588–4590. https://doi.org/10.1128/jcm.39.12.4588-4590.2001
Victor H, Ganda V, Kiranadi B, Pinontoan R (2020) Metabolite identification from biodegradation of congo red by Pichia sp. KnE Life Sci 2020:102–110. https://doi.org/10.18502/kls.v5i2.6443
Vilhena AOA, Lima KMM, Azevedo LFC, Rissino JD, Souza ACP, Nagamachi CY, Pieczarka JC (2023) The synthetic dye malachite green found in food induces cytotoxicity and genotoxicity in four different mammalian cell lines from distinct tissues. Toxicol Res 2023:1–9
Woiski C, Dobslaw D, Engesser KH (2020) Isolation and characterization of 2-butoxyethanol degrading bacterial strains. Biodegradation 31:153–169. https://doi.org/10.1007/s10532-020-09900-3
Yang C, Ho YN, Makita R, Inoue C, Chien MF (2020) Cupriavidus basilensis strain r507, a toxic arsenic phytoextraction facilitator, potentiates the arsenic accumulation by Pteris vittata. Ecotoxicol Environ Saf 190:1–8. https://doi.org/10.1016/j.ecoenv.2019.110075
Zerva A, Pentari C, Termentzi A, America AHP, Zouraris D, Bhattacharya SK, Karantonis A, Zervakis GI, Topakas E (2021) Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis. Biotechnol Biofuels 14:1–16. https://doi.org/10.1186/s13068-021-01937-7
Zhang H, Liu C, Zhang X, Yanga H, Sun J, Liu C, Li N (2022) The key sulfometuron-methyl degrading bacteria isolation based on soil bacterial phylogenetic molecular ecological networks and application for bioremediation of contaminated soil by immobilization. Ecotoxicol Environ Saf 238:1–10. https://doi.org/10.1016/j.ecoenv.2022.113605
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This work was supported by The Indonesian Ministry of Education, Directorate General of Higher Education, Research, and Technology (DIKTIRISTEK) (1218/LL3/PG/2021).
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Formal analysis and investigation were performed by JSP. Methodology was planned by HV. Conceptualization and supervision were performed by RP. Funding acquisition was performed by RP, MC, and HV. The first draft of the manuscript was written by JSP, HV, and RP. JSP, HV, D, MC, and RP have reviewed, edited, and approved the final manuscript.
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Purnomo, J.S., Victor, H., Dikson et al. Decolorization potential of malachite green by Ralstonia mannitolilytica isolated from Indonesian cassava-based fermented food tapai. Arch Microbiol 205, 339 (2023). https://doi.org/10.1007/s00203-023-03678-7
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DOI: https://doi.org/10.1007/s00203-023-03678-7