Abstract
Known for its species abundance and evolutionary status complexity, family Roseobacteraceae is an important subject of many studies on the discovery, identification, taxonomic status, and ecological properties of marine bacteria. This study compared and analyzed the phylogenetic, genomic, biochemical, and chemo taxonomical properties of seven species from three genera (Psychromarinibacter, Lutimaribacter, and Maritimibacter) of the family Roseobacteraceae. Moreover, a novel strain, named C21-152T was isolated from solar saltern sediment in Weihai, China. The values of 16S rRNA gene sequence similarity, the average nucleotide identity (ANI), the average amino acid identity (AAI), and the digital DNA–DNA hybridization (dDDH) between genomes of the novel strain and Psychromarinibacter halotolerans MCCC 1K03203T were 97.19, 78.49, 73.45, and 21.90%, respectively. Genome sequencing of strain C21-152T revealed a complete Sox enzyme system related to thiosulfate oxidization as well as a complete pathway for the final conversion of hydroxyproline to α-ketoglutarate. In addition, strain C21-152T was resistant to many antibiotics and had the ability to survive below 13% salinity. This strain had versatile survival strategies in saline environments including salt-in, compatible solute production and compatible solute transport. Some of its physiological features enriched and complemented the knowledge of the characteristics of the genus Psychromarinibacter. Optimum growth of strain C21-152T occurred at 37 ℃, with 5–6% (w/v) NaCl and at pH 7.5. According to the results of the phenotypic, chemotaxonomic characterization, phylogenetic properties and genome analysis, strain C21-152T should represent a novel specie of the genus Psychromarinibacter, for which the name Psychromarinibacter sediminicola sp. nov. is proposed. The type strain is C21-152T (= MCCC 1H00808T = KCTC 92746T = SDUM1063002T).
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Data availability
The GenBank accession number for 16S rRNA gene sequence of strain C21-152T is ON954837 and the draft genome has been deposited in GenBank under the accession number JARGYC000000000. The draft genome of P. halotolerans MCCC 1K03203T has been deposited in GenBank with the ac-cession number JARGYD000000000.
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Acknowledgements
The scanning electron microscopy was supported by the Physical-Chemical Materials Analytical and Testing Center of Shandong University at Weihai.
Funding
This work was funded by the National Natural Science Foundation of China (32200003), Natural Science Foundation of Shandong Province (ZR2022QC106), Guangdong Basic and Applied Basic Research Foundation (2022A1515110773) and the National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700).
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Y-HW performed material preparation and isolated strain C21-152T. Y-HW, J-CL and J-HX performed experimental operation, data collection. Y-HD performed a genetic analysis. Y-HW wrote the manuscript. M-QY performed project guidance and critical revision of manuscripts. Z-JD and M-QY participated in funding acquisition, project administration and supervision. All authors contributed to the article and approved the submitted version.
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Wang, YH., Liu, JC., Du, YH. et al. Psychromarinibacter sediminicola sp. nov., a novel moderately halophilic, metabolically diverse bacterium isolated from a solar saltern sediment, and comparison between members of family Roseobacteraceae. Arch Microbiol 205, 331 (2023). https://doi.org/10.1007/s00203-023-03672-z
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DOI: https://doi.org/10.1007/s00203-023-03672-z