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Broad spectrum bioactivity of a novel β-glucan rich heteropolysaccharide, Pestalopine isolated from endophytic fungi Pestalotiopsis chamaeropsis CEL6

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Abstract

A biologically potent exopolysaccharide (EPS), Pestalopine was produced by Pestalotiopsis chamaeropsis CEL6, an endophytic fungal isolate of Chloranthus elatior Sw. Pestalopine is composed of glucose, arabinose, fucose, rhamnose, and galactose in a molar ratio of nearly 10:1:2:2:4 having an Mw ∼ 3.29 × 105 Da. Pestalopine exhibited a radical scavenging effect and significantly increased antioxidant parameters (malondialdehyde, superoxide dismutase, glutathione peroxidase, reduced glutathione oxidized glutathione) in peritoneal macrophage cells in a concentration-dependent manner, with a maximum effect at 500 mg mL−1. Pestalopine is hepatoprotective in nature and improves the liver function profiles—total bilirubin, direct bilirubin, hepatic enzymes: alkaline phosphatase, gamma-glutamyl transferase, aspartate aminotransferase, alanine transaminase of liver fibrosis induced (through thioacetamide) male Wistar rats in comparison to control. Pestalopine-fed rats are reported to have higher counts of beneficial Lactobacillus sp. Present findings suggest that Pestalopine, a novel compound may have promise as a non-toxic exogenous antioxidant with hepatoprotective and probiotic efficacies.

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Authors and Affiliations

  1. Microbiology and Microbial Biotechnology Laboratory, Department of Botany and Forestry, Vidyasagar University, Midnapore, 721102, West Bengal, India

    Hiran Kanti Santra & Debdulal Banerjee

  2. Center for Life Sciences, Vidyasagar University, Midnapore, 721102, West Bengal, India

    Debdulal Banerjee

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  1. Hiran Kanti Santra
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HKS performed the work and wrote the manuscript. DB supervised the planning of the project and critically reviewed the manuscript.

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Correspondence to Debdulal Banerjee.

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Communicated by Yusuf Akhter.

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Santra, H.K., Banerjee, D. Broad spectrum bioactivity of a novel β-glucan rich heteropolysaccharide, Pestalopine isolated from endophytic fungi Pestalotiopsis chamaeropsis CEL6. Arch Microbiol 205, 284 (2023). https://doi.org/10.1007/s00203-023-03620-x

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