Abstract
Hahella is a genus that has not been well-studied, with only two identified species. The potential of this genus to produce cellulases is yet to be fully explored. The present study isolated Hahella sp. CR1 from mangrove soil in Tanjung Piai National Park, Malaysia, and performed whole genome sequencing (WGS) using NovaSeq 6000. The final assembled genome consists of 62 contigs, 7,106,771 bp, a GC ratio of 53.5%, and encoded for 6,397 genes. The CR1 strain exhibited the highest similarity with Hahella sp. HN01 compared to other available genomes, where the ANI, dDDH, AAI, and POCP were 97.04%, 75.2%, 97.95%, and 91.0%, respectively. In addition, the CAZymes analysis identified 88 GTs, 54 GHs, 11 CEs, 7 AAs, 2 PLs, and 48 CBMs in the genome of strain CR1. Among these proteins, 11 are related to cellulose degradation. The cellulases produced from strain CR1 were characterized and demonstrated optimal activity at 60 ℃, pH 7.0, and 15% (w/v) sodium chloride. The enzyme was activated by K+, Fe2+, Mg2+, Co2+, and Tween 40. Furthermore, cellulases from strain CR1 improved the saccharification efficiency of a commercial cellulase blend on the tested agricultural wastes, including empty fruit bunch, coconut husk, and sugarcane bagasse. This study provides new insights into the cellulases produced by strain CR1 and their potential to be used in lignocellulosic biomass pre-treatment.
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Data availability
The 16S rRNA partial gene sequence obtained from Sanger sequencing can be retrieved in GenBank (accession number OQ607710). The raw reads from the Illumina sequencing were deposited in NCBI Sequence Read Archive (SRA) database with accession number SRR22726834. The genome assembly can be retrieved from the NCBI database under the accession number JAPELK000000000 (BioProject ID: PRJNA896735; BioSample ID: SAMN31564167).
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Acknowledgements
M.C.Y.T. would like to thank UTMNexus of Universiti Teknologi Malaysia for scholarship support. K.J.L. is a researcher under the Post-Doctoral Fellowship Scheme provided by Universiti Teknologi Malaysia.
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This work was supported by the Ministry of Higher Education Malaysia under Fundamental Research Grant Scheme (FRGS/1/2019/WAB13/UTM/02/1) as main sponsor. This work was also supported by Professional Development Research University Grant (Q.J130000.21A2.06E00).
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MCYT performed the genome analysis, carried out the enzyme experiments and wrote the manuscript. MRZ collected the sequencing data. KJL reviewed and edited the manuscript. CSC conceived the project. All authors reviewed and approved the final manuscript.
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203_2023_3617_MOESM1_ESM.tif
Supplementary file1 The effect of temperature (A), pH (B) and salinity (C) on the crude cellulase activity from Hahella sp. CR1. Mean values (n=3) are expressed and standard deviations is indicated as error bars (TIF 21757 KB)
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Tan, M.C.Y., Zakaria, M.R., Liew, K.J. et al. Draft genome sequence of Hahella sp. CR1 and its ability in producing cellulases for saccharifying agricultural biomass. Arch Microbiol 205, 278 (2023). https://doi.org/10.1007/s00203-023-03617-6
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DOI: https://doi.org/10.1007/s00203-023-03617-6