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Morphological modification of silver nanoparticles against multi-drug resistant gram–negative bacteria and cytotoxicity effect in A549 lung cancer cells through in vitro approaches

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Abstract

In the present study, the individual cultures of Proteus mirabilis (P. mirabilis) and Klebsiella pneumoniae (K. pneumoniae) were treated with morphologically modified silver nanoparticles (Ag NPs) and were found to display zones of inhibition of ~ 8 mm, 16 mm, 20 mm, and 22 mm (P. mirabilis) and 6 mm, 14 mm, 20 mm, and 24 mm (K. pneumoniae) at concentrations of 25 µg/ml, 50 µg/mL, 75 µg/mL, and 100 µg/mL, respectively. In addition, turbidity tests were performed based on O. D. values, which exhibited 92% and 90% growth inhibitions at 100 µg/mL concentration for P. mirabilis and K. pneumoniae, respectively. Furthermore, the IC50 concentration of Ag NPs was established for A549 lung cancer cells and found to be at 500 µg/mL. Evidently, the morphological variation of Ag NPs treated A549 lung cancer cells was exhibited with differential morphology studied by phase-contrast microscopy. The results demonstrated that the synthesized Ag NPs was not only efficient against gram-positive bacteria but also against gram-negative bacteria and A549 cancer cells, suggesting that the potential of these biosynthesized Ag NPs is a future drug discovery source for inhibiting bacteria and cancer cells.

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Acknowledgements

G. Rajivgandhi and Franck Quero acknowledge the financial support from ANID-FONDECYT (Chile) under the Postdoctoral Fellowship No. 3220019. The authors extend their appreciation to the Researchers Supporting Project number (RSP2023R70), King Saud University, Riyadh, Saudi Arabia.

Funding

Researchers Supporting Project No. (RSP2023R70) the King Saud University, Riyadh, Saudi Arabia. G. Rajivgandhi and Franck Quero acknowledge the financial support from ANID-FONDECYT (Chile) under the Postdoctoral Fellowship No. 3220019.

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Authors and Affiliations

  1. Laboratorio de Nanocelulosa y Biomateriales, Departamento de Ingeniería Química, Biotecnología y Materiales, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile, Avenida Beauchef 851, Santiago, 8370456, Chile

    Govindan Rajivgandhi & Franck Quero

  2. Department of Nanotechnology, Noorul Islam Centre for Higher Education, Kumaracoil, Kanyakumari, Tamil Nadu, 629180, India

    Chenthis Kanisha Chelliah

  3. Department of Marine Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India

    Govindan Rajivgandhi, Govindan Ramachandran, Gnansekaran Chackaravarthi & Manoharan Natesan

  4. Lab of Toxicology, Department of Health Sciences, The Graduate School of Dong-A University, 37, Nakdong-Dearo 550 Beon-Gil, Saha-Gu, Busan, 49315, South Korea

    Muthuchamy Maruthupandy

  5. Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia

    Naiyf S. Alharbi & Shine Kadaikunnan

  6. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Govindan Rajivgandhi & Wen-Jun Li

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  1. Govindan Rajivgandhi
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  2. Chenthis Kanisha Chelliah
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  3. Govindan Ramachandran
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  5. Muthuchamy Maruthupandy
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  7. Shine Kadaikunnan
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  9. Wen-Jun Li
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  10. Franck Quero
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Contributions

GR and FQ designed and done the nanoparticles and anti-bacterial activities experiments. GR and GC contributed in the in vitro inhibition experiments for bacteria. CKC, MM, NA, SK and MN contributed in the entire biological application process and also written the manuscript, corrected the grammatical errors, language in throughout of the manuscript. W-JL contributed entire work of the manuscript. He contributed in the structural arrangement, graphical representation and language corrections in throughout of the manuscript.

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Correspondence to Muthuchamy Maruthupandy or Franck Quero.

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All the authors report no any conflicts of interest.

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Communicated by Arivalagan Pugazhendhi.

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Rajivgandhi, G., Chelliah, C.K., Ramachandran, G. et al. Morphological modification of silver nanoparticles against multi-drug resistant gram–negative bacteria and cytotoxicity effect in A549 lung cancer cells through in vitro approaches. Arch Microbiol 205, 282 (2023). https://doi.org/10.1007/s00203-023-03611-y

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