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Deposition of Daldinia starbaeckii (ELF) functionalized silver nanoparticles on urinary catheter tube using chitosan polymer to prevent microbial biofilms formation during UTI infection

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Abstract

Catheter-associated urinary tract infections (CAUTI) are the most common healthcare problem in hospitals. In this study, we isolated the Daldinia starbaeckii (An endolichenic fungus from Roccella montagnie) and its biomass extract were used to simultaneously synthesize and deposit DSFAgNPs on the inner and outer surfaces of the catheter tube using chitosan biopolymer via In-situ deposition method. Perfectly designed D. starbaeckii extract functionalized DSFAgNPs were characterized by UV spectroscopy, FTIR, SEM, EDS, TEM, and XRD. The microbial efficacy of DSFAgNPs & DSFAgNPs coated catheter (CTH3) was evaluated against eight human pathogenic gram (+ / −) ive strains and Candida albicans. Results indicated DSFAgNPs showed significant biological activity against both gram (+ / −) ive bacteria with an average MIC90 of 4 µl/ml. The most promising activity was observed against Helicobacter pylori. When bacteria strains allow to grow with CTH3 we reported significant reduction in colony formation unit (CFU/ml) in broth culture assay with an average 70% inhibition. Further, antibiofilm activity of CTH3 against P. aeruginosa showed strong inhibition of biofilm formation (85%). The study explored an alternate approach for significantly prevent CAUTI among hospital patients.

Graphical abstract

We isolated an endolichenic fungus from lichen Roccella montagnei. The molecular characterization of fungus identified as Daldinia starbaeckii (DSF). The DSF was cultured and its fungal biomass exudes were used to simultaneously construct DSF-AgNPs and its deposition on the catheter surface using biopolymer chitosan via In-situ deposition method. Further, antimicrobial and antibiofilm efficacy of DSF-AgNPs was checked against urinary catheter contaminating and human pathogenic bacterial strains. Based on our research, we determined that DSF-AgNPs coating on a urinary catheter through this method is a cost-effective, eco-friendly approach to prevent catheter contamination.

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Data availability

The sequence data generated during the current study of Daldinia starbaeckii are available in the Genbank repository under accession numbers OM095441.

Abbreviations

EDAX:

Energy Dispersive X-ray Analysis

CAUTI:

Catheter-associated urinary tract infections

ELF:

Endolichenic fungus

DSF:

Daldinia starbaeckii Fungus

MIC:

Minimum inhibitory concentration

CFU:

Colony forming unit

RT:

Room temperature

NPs:

Nanoparticles

ZOP:

Zone of Prohibition

QS:

Quorum sensing

rpm:

Rotation per minute

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Acknowledgements

The authors are thankful to the Director, CSIR- National Botanical Research Institute, Lucknow for providing laboratory facilities and the Vice Chancellor, BBAU, Lucknow for providing research-oriented environment. The author is also grateful to Prof. Rajesh Kumar, my supervisor/mentor, who always helped and encouraged me to achieve any research goals.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Lichenology Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Shweta Bharti & Sanjeeva Nayaka

  2. Department of Microbiology, Babasaheb Bhimrao Ambedkar Central University, Lucknow, 226025, India

    Shweta Bharti & Rajesh Kumar

  3. Department of Biotechnology, Bundelkhand University, Jhansi, 284128, India

    Balwant Singh Paliya

Authors
  1. Shweta Bharti
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  2. Balwant Singh Paliya
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  3. Sanjeeva Nayaka
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  4. Rajesh Kumar
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Contributions

Shweta Bharti: Conceptualization, Investigation, Writing - Original Draft, Data Curation, Formal analysis, B.S. Paliya: Writing - Review & Editing, Visualization. Sanjeeva Nayka: Supervision Validation, Review & Editing Rajesh Kumar: Supervision, Conceptualization, Editing.

Corresponding author

Correspondence to Rajesh Kumar.

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Communicated by Gharieb El-Sayyad.

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Bharti, S., Paliya, B.S., Nayaka, S. et al. Deposition of Daldinia starbaeckii (ELF) functionalized silver nanoparticles on urinary catheter tube using chitosan polymer to prevent microbial biofilms formation during UTI infection. Arch Microbiol 205, 277 (2023). https://doi.org/10.1007/s00203-023-03608-7

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