Skip to main content
SpringerLink
Log in

Functional characterization of two homologs of yeast acetyl-coenzyme A synthetase in the entomopathogenic fungus Beauveria bassiana

  • Original Paper
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Acetyl-coenzyme A (CoA) synthetase (Acs) links cellular metabolism and physiology by catalyzing acetate and CoA into acetyl-CoA. However, the biological roles of Acs are not well studied in entomopathogenic fungi. In this study, two Acs proteins (BbAcs1 and BbAcs2) was functionally characterized in the filamentous insect pathogenic fungus Beauveria bassiana. BbAcs1 and BbAcs2 localize in cytoplasm and peroxisome, respectively. BbAcs1 contributes to vegetative growth on fatty acids as carbon source, and BbAcs2 did not. Both genes did not contribute to fungal response to stresses. The BbAcs1 loss conferred a slight influence on conidiation, and did not result in the defects in blastospore formation. On the contrary, BbAcs2 significantly contributes to lipid metabolism in germlings, blastospore formation, and virulence. The results indicated that Acs2 played a more predominant role than Acs1 in B. bassiana, which links the acetyl-CoA metabolism with the lifestyle of entomopathogenic fungi.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Chen Y, Siewers V, Nielsen J (2012) Profiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae. PLoS ONE 7:e42475

    Article  CAS  Google Scholar 

  • De Cima S, Rúa J, Perdiguero E, del Valle P, Busto F, Baroja-Mazo A, de Arriaga D (2005) An acetyl-CoA synthetase not encoded by the facA gene is expressed under carbon starvation in Phycomyces blakesleeanus. Res Microbiol 156:663–669

    Article  Google Scholar 

  • Ding JL, Hou J, Feng MG, Ying SH (2020) Transcriptomic analyses reveal comprehensive responses of insect hemocytes to mycopathogen Beauveria bassiana, and fungal virulence-related cell wall protein assists pathogen to evade host cellular defense. Virulence 11:1352–1365

    Article  CAS  Google Scholar 

  • Ding JL, Hou J, Li XH, Feng MG, Ying SH (2021) Transcription activator Swi6 interacts with Mbp1 in MluI cell cycle box-binding complex and regulates hyphal differentiation and virulence in Beauveria bassiana. J Fungi (basel) 7:411

    Article  CAS  Google Scholar 

  • Ding JL, Peng YJ, Chu XL, Feng MG, Ying SH (2018) Autophagy-related gene BbATG11 is indispensable for pexophagy and mitophagy, and contributes to stress response, conidiation and virulence in the insect mycopathogen Beauveria bassiana. Environ Microbiol 20:3309–3324

    Article  CAS  Google Scholar 

  • Falcón AA, Chen S, Wood MS, Aris JP (2010) Acetyl-coenzyme A synthetase 2 is a nuclear protein required for replicative longevity in Saccharomyces cerevisiae. Mol Cell Biochem 333:99–108

    Article  Google Scholar 

  • Galdieri L, Zhang T, Rogerson D, Lleshi R, Vancura A (2014) Protein acetylation and acetyl coenzyme a metabolism in budding yeast. Eukaryot Cell 13:1472–1483

    Article  Google Scholar 

  • Gauthier GM (2015) Dimorphism in fungal pathogens of mammals, plants, and insects. PLoS Pathog 11:e1004608

    Article  Google Scholar 

  • Gu Q, Yuan Q, Zhao D, Huang J, Hsiang T, Wei Y, Zheng L (2019) Acetyl-coenzyme A synthetase gene ChAcs1 is essential for lipid metabolism, carbon utilization and virulence of the hemibiotrophic fungus Colletotrichum higginsianum. Mol Plant Pathol 20:107–123

    Article  CAS  Google Scholar 

  • Karan D, David JR, Capy P (2001) Molecular evolution of the AMP-forming Acetyl-CoA synthetase. Gene 265:95–101

    Article  CAS  Google Scholar 

  • Klein HP, Jahnke L (1979) Effects of aeration on formation and localization of the acetyl coenzyme A synthetase of Saccharomyces cerevisiae. J Bacteriol 137:179–184

    Article  CAS  Google Scholar 

  • Lee S, Son H, Lee J, Min K, Choi GJ, Kim JC, Lee YW (2011) Functional analyses of two acetyl coenzyme A synthetases in the ascomycete Gibberella zeae. Eukaryot Cell 10:1043–1052

    Article  CAS  Google Scholar 

  • Lin HY, Pang MY, Feng MG, Ying SH (2022) A peroxisomal sterol carrier protein 2 Scp2 contributes to lipid trafficking in differentiation and virulence of the insect pathogenic fungus Beauveria bassiana. Fungal Genet Biol 158:103651

    Article  CAS  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402–408

    Article  CAS  Google Scholar 

  • Ortiz-Urquiza A, Keyhani NO (2013) Action on the surface: entomopathogenic fungi versus the insect cuticle. Insects 4:357–374

    Article  Google Scholar 

  • Ortiz-Urquiza A, Keyhani NO (2016) Molecular genetics of Beauveria bassiana infection of insects. Adv Genet 94:165–249

    Article  CAS  Google Scholar 

  • Peng YJ, Wang JJ, Lin HY, Ding JL, Feng MG, Ying SH (2020) HapX, an indispensable bZIP transcription factor for iron acquisition, regulates infection initiation by orchestrating conidial oleic acid homeostasis and cytomembrane functionality in mycopathogen Beauveria bassiana. mSystems 5:e00695-20

  • Peng YJ, Zhang H, Feng MG, Ying SH (2022) Steryl acetyl hydrolase 1 (BbSay1) links lipid homeostasis to conidiogenesis and virulence in the entomopathogenic fungus Beauveria bassiana. J Fungi (basel) 8:292

    Article  CAS  Google Scholar 

  • Pieuchot L, Jedd G (2012) Peroxisome assembly and functional diversity in eukaryotic microorganisms. Annu Rev Microbiol 66:237–263

    Article  CAS  Google Scholar 

  • Starai VJ, Escalante-Semerena JC (2004) Acetyl-coenzyme A synthetase (AMP forming). Cell Mol Life Sci 61:2020–2030

    Article  CAS  Google Scholar 

  • Takahashi H, McCaffery JM, Irizarry RA, Boeke JD (2006) Nucleocytosolic acetyl-coenzyme A synthetase is required for histone acetylation and global transcription. Mol Cell 23:207–217

    Article  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    Article  CAS  Google Scholar 

  • van den Berg MA, de Jong-Gubbels P, Kortland CJ, van Dijken JP, Pronk JT, Steensma HY (1996) The two acetylcoenzymeA synthetases of Saccharomyces cerevisiae differ with respect to kinetic properties and transcriptionalregulation. J Biol Chem 271:28953–28959

    Article  Google Scholar 

  • van den Berg MA, de Jong-Gubbels P, Kortland CJ, van Dijken JP, Pronk JT, Steensma HY (2004) The two acetyl-coenzyme A synthetases of Saccharomyces cerevisiae differ with respect to kinetic properties and transcriptional regulation. J Biol Chem 271:28953–28959

    Article  Google Scholar 

  • van den Berg MA, Steensma HY (1995) ACS2, a Saccharomyces cerevisiae gene encoding acetyl-coenzyme A synthetase, essential for growth on glucose. Eur J Biochem 231:704–713

    Article  Google Scholar 

  • Wang CS, Feng MG (2014) Advances in fundamental and applied studies in China of fungal biocontrol agents for use against arthropod pests. Biol Control 68:129–135

    Article  Google Scholar 

  • Wang JJ, Peng YJ, Ding JL, Feng MG, Ying SH (2020) Mitochondrial fission is necessary for mitophagy, development and virulence of the insect pathogenic fungus Beauveria bassiana. J Appl Microbiol 129:411–421

    Article  CAS  Google Scholar 

  • Ying SH, Liu J, Chu XL, Xie XQ, Feng MG (2016) The autophagy-related genes BbATG1 and BbATG8 have different functions in differentiation, stress resistance and virulence of mycopathogen Beauveria bassiana. Sci Rep 6:26376

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The author thanks Dr. She-Long Zhang (Equipment and Technology Service Platform, College of Life Sciences, Zhejiang University) for his help with fluorescence microscopy.

Funding

This research was supported by the National Natural Science Foundation of China (32170027 and 31972331) (S.Y.).

Author information

Authors and Affiliations

  1. Institute of Microbiology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China

    Jia-Hui Lei, Hai-Yan Lin, Jin-Li Ding, Ming-Guang Feng & Sheng-Hua Ying

Authors
  1. Jia-Hui Lei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hai-Yan Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jin-Li Ding
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ming-Guang Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sheng-Hua Ying
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Jia-Hui Lei, Hai-Yan Lin, and Jin-Li Ding. The first draft of the manuscript was written by Jia-Hui Lei and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Sheng-Hua Ying.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Communicated by Erko Stackebrandt.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 497 KB)

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lei, JH., Lin, HY., Ding, JL. et al. Functional characterization of two homologs of yeast acetyl-coenzyme A synthetase in the entomopathogenic fungus Beauveria bassiana. Arch Microbiol 204, 653 (2022). https://doi.org/10.1007/s00203-022-03269-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00203-022-03269-y

Keywords

Search

Navigation