Abstract
A novel aerobic, Gram-staining-negative, non-motile, short-rod-shaped strain, designated f23T, was obtained from Daihai Lake, Inner Mongolia, Republic of China. 16S rRNA gene sequences analysis showed that f23T belongs to the genus Orrella and is most closely related to Orrella marina H-Z20T with 98.35% sequence similarity. The strain was oxidase positive, catalase positive and had well growth at pH 6.5–8.5, at temperature 28–40 °C and at 0–4.5% (w/v) NaCl. Colonies incubated at 37 °C on marine 2216 agar for 3 days were white, smooth, transparent, circular and less than 1.0 mm in diameter. The total genome size of f23T was 2,803,849 bp with a G + C content of 52.79%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain f23T and O. marina H-Z20T were 69.62% and 20.5%, which both below the species delineation threshold. Chemotaxonomic analysis showed that C16:0, cyclo-C17:0, C18:0, Sum Feature 3 (C16:1ω7c and/or C16:1ω6c) and Sum Feature 8 (C18:1ω6c and C18:1ω7c) as the major fatty acids, ubiquinone-8 as the major isoprenoid quinone, phosphatidylethanolamine as the major cellular polar lipids. Based on the polyphasic analysis, f23T represents a novel species within the genus Orrella, for which the name Orrella daihaiensis sp. nov. is proposed. The type strain is f23T (= CGMCC 1.18761 T = KCTC 82425 T).
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Availability of data and materials
The GenBank accession numbers for the whole genome sequence and the 16S rRNA gene sequence of the type stain f23T are CP063982 and MW179610. The supplementary material is available online.
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Abbreviations
- MA:
-
Marine 2216 agar
- MB:
-
Marine 2216 broth
- LB:
-
Luria-Bertrani
- ML:
-
Maximum-likelihood
- NJ:
-
Neighbour-joining
- ME:
-
Minimum-Evolution
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA-DNA hybridization
- GGDC:
-
Genome-to-Genome Distance Calculator
- DPG:
-
Diphosphatidylglycerol
- PG:
-
Phosphatidylglycerol
- PE:
-
Phosphatidylethanolamine
- MCCC:
-
Marine Culture Collection of China
- CGMCC:
-
China General Microbiological Culture Collection Center
- KCTC:
-
The Korean Collection for Type Cultures
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Acknowledgements
We really thank Dr. Lei Zhai in the China center of industrial culture collection (CICC) for the help on the investigation of cellular fatty acids profiles of strain f23T and reference strain.
Funding
This work was supported by the Natural Science Foundation of Inner Mongolia (No.2020BS03006), and the scientific research starting funding of Inner Mongolia Normal University (No. 2018YJRC015).
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Conceptualization: K.J and B.Y.; funding acquisition: K.J.; resource: K.J and C.L.C.; investigation: K.J., C.L.C., B.Y., R.X.L., Y.L., C.Y.Z., X.H.H., K.Y.Q and H.Z.Y. writing—original draft: K.J.; writing—review and editing: C.L.C.; all of the authors approved the final version of the manuscript.
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Jiang, K., Yuan, B., Cao, C. et al. Orrella daihaiensis sp. nov., a bacterium isolated from Daihai Lake in Inner Mongolia. Arch Microbiol 204, 427 (2022). https://doi.org/10.1007/s00203-022-03056-9
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DOI: https://doi.org/10.1007/s00203-022-03056-9