Abstract
Thurincin H is a bacteriocin produced by Bacillus thuringiensis, it is encoded in a group of ten genes, most of which have been characterized experimentally or by homology. However, the activity of the thnI gene encoding a 95 amino acid ORF remains unknown. In this work, the thnI gene was cloned under the regulation of two promoters and transformed into a sensitive strain to determine if it acts as an immunity protein. In addition, a deletion mutant without the thnI gene was used to test whether thnI is required or not for the biosynthesis of thurincin H. It was concluded that thnI does not provide immunity and is not required to produce thurincin H.
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Funding
Luz E. Casados-Vázquez is a Young Associate Research supported by “Consejo Nacional de Ciencia y Tecnología” (CONACYT), México (Grant 269). This research was supported by Grant “Problemas Nacionales 2016” CONACyT (3664) and M. A. Núñez-Valle is a Bioscience graduate student supported by CONACyT. We thank to the Laboratorio Nacional PlanTECC, México, for the financial support granted in the project ‘Mantenimiento de la infraestructura del Laboratorio Nacional PlanTECC’.
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Núñez-Valle and Casados-Vázquez carried out the experiment. Casados-Vázquez and Massange-Sánchez wrote the manuscript with support from Barboza-Corona. Casados-Vázquez, Massange-Sánchez and Barboza-Corona supervised the project. Casados-Vázquez conceived the original idea.
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Massange-Sánchez, J.A., Núñez-Valle, M.A., Barboza-Corona, J.E. et al. The thnI gene is not required for thurincin H biosynthesis or immunity. Arch Microbiol 204, 344 (2022). https://doi.org/10.1007/s00203-022-02938-2
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DOI: https://doi.org/10.1007/s00203-022-02938-2