Abstract
We studied the survival of four elite strains of Bradyrhizobium in liquid inoculants with three formulations with exopolysaccharides extracted from other rhizobia genera, and the symbiotic efficiency of these elite strains with soybean and cowpea in a greenhouse. For that purpose, we verified the effectiveness of formulations for maintaining the cell viability of strains by counting the colony-forming units (CFU) per milliliter of the liquid inoculants with formulations after 90 days. For survival of the soybean inoculant strains, 29W and CPAC15, the largest number of CFU (> 1010 mL−1) after 90 days was observed in the PEPS formulation. For the cowpea inoculant strains, INPA3-11B and UFLA3-84, the REPS1 formulation had the largest number of CFU (> 1010 mL−1) after 90 days. The symbiotic efficiency of the PEPS formulation, followed by REPS2, was higher than that shown by the commercial inoculant in soybean. For cowpea, the three formulations with EPS, especially REPS1, showed symbiotic efficiency better than that of the commercial inoculant.
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Funding
We wish to thank the Programa de Bolsa de Incentivo à Qualificação dos Servidores do Instituto Federal de Educação, Ciência e Tecnologia do Maranhão (IFMA) for granting a doctoral scholarship to the first author; we are thankful for financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (CAPES/PROEX AUXPE 593/2018), to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (304527/2016-5, 431504/2016-4), for the research productivity scholarship and grant to the corresponding author, and to the Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG) for financial support.
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TPF, BLS, FMSM: conceived of study, performed research, analyzed data and wrote the paper. CSBD helped in performing research.
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Communicated by Jorge Membrillo-Hernández.
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Palhares Farias, T., Lima Soares, B., Barbosa D’Eça, C.S. et al. Polymeric formulations of liquid inoculants with rhizobia exopolysaccharides increase the survival and symbiotic efficiency of elite Bradyrhizobium strains. Arch Microbiol 204, 177 (2022). https://doi.org/10.1007/s00203-022-02779-z
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DOI: https://doi.org/10.1007/s00203-022-02779-z