Abstract
Truffles are the fruiting bodies of hypogeous fungi in the genus Tuber. Some truffle species usually grow in an area devoid of vegetation, called brûlé, but limited knowledge is available on the microbial composition and structure of them. Here, we investigated the bacterial and fungal communities of Tuber indicum ascocarps and soils inside and outside a characteristic brûlé from a poplar plantation with no truffle production history in northeastern China using a high-throughput sequencing approach. A predominance of members of the bacterial phylum Proteobacteria was observed in all samples. Members of Bacillus were the main genera in the ascocarps, while members of Lysobacter and unidentified Acidobacteria were more abundant in the soil. In addition, members of Gibberella, Fusarium, and Absidia were the dominant fungi in the ascocarps, while members of Tuber were enriched in the ascocarps and soils inside the brûlé. Some mycorrhization helper bacteria (Rhizobium) and ectomycorrhiza-associated bacteria (Lysobacter) were detected, indicating their potential roles in the complex development of underground fruiting bodies and brûlé formation. These findings may contribute to the protection and cultivation of truffles.
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Data availability statement
The datasets generated for this study are available at the NCBI database under the GenBank accession number PRJNA669952.
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Acknowledgements
This study was supported by the Project of Shandong Provincial Natural Science Foundation (ZR2020MC001), the National Natural Science Foundation of China (31200248 and 31500016), the Project of Shandong Province Higher Educational Science and Technology Program (J14LE07), and the Key Research and Development Program of Yantai Grant (2019XDHZ089). The authors thank AJE (https://www.aje.com/) for providing linguistic assistance during the preparation of this manuscript.
Funding
This study was supported by the Project of Shandong Provincial Natural Science Foundation (ZR2020MC001), the National Natural Science Foundation of China (31200248 and 31500016), the Project of Shandong Province Higher Educational Science and Technology Program (J14LE07), and the Key Research and Development Program of Yantai Grant (2019XDHZ089).
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Wang, D., Xu, Q., Guo, W. et al. Microbial communities of ascocarps and soils in a natural habitat of Tuber indicum. Arch Microbiol 204, 189 (2022). https://doi.org/10.1007/s00203-022-02763-7
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DOI: https://doi.org/10.1007/s00203-022-02763-7