Abstract
A novel Gram-stain-negative bacterium, designated as IM2376T, was isolated from the sediment of Hutong Qagan Lake in the Ordos, Inner Mongolia Autonomous Region of China. Phylogenetic analysis based on 16S rRNA gene sequence revealed that the strain IM2376T had the highest similarity with Roseinatronobacter thiooxidans DSM 13087T (96.2%) and Rhodobaca bogoriensis LBB1T (96.2%) of the family Rhodobacteraceae. Genomic relatedness analyses showed that strain IM2376T was clearly distinguished from other species in the family Rhodobacteraceae, with average nucleotide identities, average amino acid identities, and in silico DNA–DNA hybridization values not more than 74.1, 68.5, and 20.2%, respectively. The fatty acids were mainly composed of C18:1ω7c (64.9%), iso-C16:0 (16.3%), and C16: 1ω7c/C16:1ω6c (6.0%). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylcholine. The predominant ubiquinone was Q-10 (94.9%). The genomic DNA G + C content was 66 mol%. Based on all these results, strain IM2376T was considered a novel species of a new genus in the family Rhodobacteraceae, for which the name Rhabdonatronobacter sediminivivens gen. nov., sp. nov. is proposed. The type strain of Rhabdonatronobacter sediminivivens is IM2376T (= CGMCC 1.17852T = KCTC 92134T).
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Abbreviations
- DPG:
-
Diphosphatidylglycerol
- PG:
-
Phosphatidylglycerol
- PC:
-
Phosphatidylcholine
- isDDH:
-
The in silico DNA–DNA hybridization
- AAI:
-
The average amino acid identity
- ANI:
-
The average nucleotide identity
- PHA:
-
Polyhydroxyalkanoate
- PHB:
-
Polyhydroxybutyrate
- sqr :
-
Sulfide-quinone reductase gene
- fccA :
-
Cytochrome subunit of sulfide dehydrogenase gene
- fccB :
-
Sulfide dehydrogenase [flavocytochrome c] flavoprotein chain gene
- GTDB:
-
The Genome Taxonomy Database
- ML:
-
The maximum-likelihood
- NJ:
-
Neighbor-joining
- ME:
-
Minimum evolution
- TYGS:
-
The type (strain) genome server
- OPNG:
-
The O-nitrophenyl-β-d-galactopyranoside
- VP:
-
Voges Proskauer
- tmRNA:
-
Transfer-messenger-RNA
- CDS:
-
Coding sequence
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Funding
This study was funded by the National Natural Science Foundation of China (No. 91751201) and the National Science and Technology Foundation Project of China (No. 2017FY100300).
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HX designed and supervised the study. HZ, DZ, SZ, QX and JZ collected the samples. QX isolated the strain. HZ and MY performed the microbial culture and identification. HZ, DZ and SZ performed bioinformatic and statistical analyses. HZ and DZ prepared the figures and tables. HZ drafted the manuscript, and then HX, DZ, QX and SK participated in revisions. All the authors read and approved the final manuscript.
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Zhou, H., Yang, M., Xue, Q. et al. Rhabdonatronobacter sediminivivens gen. nov., sp. nov. isolated from the sediment of Hutong Qagan Soda Lake. Arch Microbiol 204, 145 (2022). https://doi.org/10.1007/s00203-022-02758-4
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DOI: https://doi.org/10.1007/s00203-022-02758-4