Abstract
A novel Gram-negative strain, designated X100-76T, was isolated from Daihai Lake in Inner Mongolia, Republic of China. The strain was non-motile, non-spore-forming, long-rod-shaped, oxidase positive and catalase positive. Colonies incubated at 33 °C on 2216 marine agar medium for 3 days were circular, smooth, transparent, convex with clear edges, orange-red in colour and approximately 1.0 mm in diameter. Growth occurred at pH 6.5–12 (optimum pH 7.5), in 0–5.0% (w/v) NaCl (optimum 1.5%, w/v) and at 4–40 °C (optimum 28–33 °C). Phylogenetic analysis based on 16S rRNA gene sequences showed that X100-76T belongs to the genus Mongoliitalea and is most closely related to Mongoliitalea lutea MIM18T with 98.3% sequence similarity. The total genome size of X100-76T was 4,816,617 bp with a G + C content of 39.6%. The digital DNA–DNA hybridization and average nucleotide identity values between strain X100-76T and M. lutea MIM18T were both below the recommended cut-off values. Chemotaxonomic analysis revealed iso-C15:0, Sum Feature 4 (anteiso-C17:1 B and/or iso-C17:1 I), C15:1ω6c, Sum Feature 9 (C16:0 10-methyl and/or iso-C17:1ω9c), C16:0 and iso-C15:1 G as the major fatty acids, menaquinone MK-7 as the major isoprenoid quinone, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol as the major cellular polar lipids. The results of polyphasic analysis demonstrated that X100-76T represents a novel species within the genus Mongoliitalea, for which the name Mongoliitalea daihaiensis sp. nov. is proposed. The type strain is X100-76T (= CGMCC 1.18762T = KCTC 82458T).
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Availability of data and material
The GenBank accession numbers for the 16S rRNA gene sequence and the whole genome sequence of the type stain X100-76T are MW179611 and CP063779. The supplementary material is available online.
Code availability
Not applicable.
Abbreviations
- CGMCC:
-
China General Microbiological Culture Collection Center
- KCTC:
-
The Korean Collection for Type Cultures
- ACCC:
-
Agricultural Culture Collection of China
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization
- MA:
-
Marine 2216 agar
- MB:
-
Marine 2216 broth
- LB:
-
Luria–Bertrani
- ML:
-
Maximum-likelihood
- NJ:
-
Neighbour-joining
- ME:
-
Minimum-evolution
- GGDC:
-
Genome-to-genome distance calculator
- DPG:
-
Diphosphatidylglycerol
- PG:
-
Phosphatidylglycerol
- PE:
-
Phosphatidylethanolamine
- PC:
-
Phosphatidylcholine
- PI:
-
Phosphatidylinositol
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Acknowledgements
We really thank Dr. Lei Zhai for the help on the investigation of cellular fatty acids profiles of strain X100-76T and reference strain.
Funding
This work was supported by the National Natural Science Foundation of China (No. 31760025), the Natural Science Foundation of Inner Mongolia (No. 2020BS03006), and the scientific research starting funding of Inner Mongolia Normal University (No. 2018YJRC015).
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Conceptualization: KJ and BY; funding acquisition: KJ and BY; resource: KJ and CLC; investigation: KJ, CLC, BY, RXL, YA and CYZ. Writing-original draft: KJ; writing-review and editing: BY and CLC; all of the authors approved the final version of the manuscript.
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Jiang, K., Yuan, B., Cao, C.L. et al. Mongoliitalea daihaiensis sp. nov., isolated from Daihai Lake in Inner Mongolia. Arch Microbiol 204, 92 (2022). https://doi.org/10.1007/s00203-021-02724-6
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DOI: https://doi.org/10.1007/s00203-021-02724-6