Abstract
A Gram-negative, rod-shaped, motile bacterium, designated strain CAU 1620T, was isolated from a tidal flat sediment in Incheon, Republic of Korea. Strain CAU 1620T grew optimally at 30 °C and pH 8.0 in the presence of 6.0% (w/v) NaCl. The results of 16S rRNA gene sequence analysis revealed that strain CAU 1620T showed the highest similarity to Marinobacter adhaerens DSM 23420T (98.5%), followed by Marinobacter algicola DSM 16394T (98.3%) and Marinobacter maroccanus LMG 30465T (98.2%). The average nucleotide identity and digital DNA–DNA hybridisation values between strain CAU 1620T and related strains were estimated as 75.6–78.1% and 19.5–20.9%, respectively. The DNA G + C content based on the draft genome sequence was 59.2%, and the major respiratory quinone was ubiquinone-9. The predominant cellular fatty acids were C12:0, C16:0, C18:1 ω9c, and C12:0 3OH. The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. According to phenotypic, genotypic, and chemotaxonomic analyses, strain CAU 1620T represents a novel species of the genus Marinobacter, for which the name Marinobacter arenosus sp. nov. is proposed. The type strain is CAU 1620T (= KCTC 82431T = MCCC 1K06079T).
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Acknowledgements
This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201902203), and the Chung-Ang University Research Grants in 2020.
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Communicated by Erko Stackebrandt.
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The 16S rRNA gene sequence and the whole-genome sequence of strain CAU 1620T have been deposited in GenBank/EMBL/DDBJ as the accession numbers MW019609 and JAHVAO000000000, respectively.
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Lee, Y., Weerawongwiwat, V., Kim, JH. et al. Marinobacter arenosus sp. nov., a halotolerant bacterium isolated from a tidal flat. Arch Microbiol 204, 155 (2022). https://doi.org/10.1007/s00203-021-02696-7
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DOI: https://doi.org/10.1007/s00203-021-02696-7