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Isolation and partial characterization of a novel bacteriocin from Pseudomonas azotoformans with antimicrobial activity against Pasterella multocida

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Abstract

In this study, a bacteriocin PA996 isolated from Pseudomonas azotoformans (P. azotoformans) was purified to homogeneity by ammonium sulphate precipitation and SP-Sepharose column chromatography. P. azotoformans began to grow at 6 h, reached exponential phase at 12–18 h. Bacteriocin PA996 was produced at 18 h and reached a maximum level of 2400 AU/mL. The molecular mass of purified bacteriocin PA996 was estimated by SDS-PAGE and its molecular mass was approximately 50 kDa. By screening in vitro, the bacteriocin PA996 showed an antimicrobial activity against Pasteurella multocida (P. multocida). The bacteriocin PA996 showed antibacterial activity in the range of pH2–10 and it was heat labile. The inhibitory activities were diminished after treatment with proteinase K, trypsin and papain, respectively, while catalase treatment was ineffective. The minimal inhibitory concentration (MIC) and bactericidal kinetics curves showed that the bacteriocin PA996 had a good inhibitory ability against P. multocida. Our data indicate that bacteriocin PA996 could inhibit the growth of P. maltocida and it may have the potential to apply as an alternative therapeutic drug.

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Funding

This study was supported by grants from the Project supported by the National Natural Science Foundation of China (31872519), Jilin Provincial Department of Science and Technology key Science and Technology projects (20160204002NY), Natural Science Foundation of Jilin Province (20190201294JC).

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Author notes

  1. Yu Wang and Shuang Lei contributed equally to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Yu Wang, Shuang Lei, Inam Muhammad, Yi-Ming Wang, Yun-Hang Gao & Ling-Cong Kong

  2. Department of Animal Sciences, Shaheed Benazir Bhutto University Sheringal, Dir Upper, Pakistan

    Inam Muhammad

  3. College of Life Science, Jilin Agricultural University, Changchun, 130118, China

    Yu Jia, Cheng-guang He & Hong-Xia Ma

  4. The Engineering Research Center of Bioreactor and Drug Development, Ministry of Education, Jilin Agricultural University, Changchun, China

    Hong-Xia Ma

Authors
  1. Yu Wang
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  2. Shuang Lei
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  3. Inam Muhammad
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  4. Yu Jia
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  5. Cheng-guang He
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  7. Yun-Hang Gao
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  8. Ling-Cong Kong
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Contributions

YW, YJ, CH and SL participated in the conception and design of the study. YG, L-CK and MH-X analyzed the data, Y-MW, YW and, MI wrote the manuscript. All authors have read and approved the final manuscript.

Corresponding authors

Correspondence to Ling-Cong Kong or Hong-Xia Ma.

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All authors have no conflicts of interest.

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Communicated by Erko Stackebrandt.

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Wang, Y., Lei, S., Muhammad, I. et al. Isolation and partial characterization of a novel bacteriocin from Pseudomonas azotoformans with antimicrobial activity against Pasterella multocida. Arch Microbiol 204, 112 (2022). https://doi.org/10.1007/s00203-021-02639-2

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  • DOI: https://doi.org/10.1007/s00203-021-02639-2

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