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Isolation of actinobacterial endophytes from wheat sprouts as biocontrol agents to control seed pathogenic fungi

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Abstract

Seed-borne Streptomyces can transmit vertically from generation to generation and be a mutualism between the endosymbionts and hosts. The aim of this study was to isolate and characterize endophytic Streptomyces strains from wheat sprouts, and to investigate their protection against wheat seed pathogenic fungi Penicillium. Endophytic Streptomyces sp. F6 and Streptomyces sp. F39 were isolated from wheat sprouts germinated under sterile conditions. Both Streptomyces strains could produce siderophores, and showed antagonistic activities against the seed pathogenic fungi Penicillium sp. Z17. The inoculation of Streptomyces sp. F39 and F6 could protect wheat seed germination and promote seedling growth under Penicillium sp. Z17 infection. However, the protection efficiency was impacted by the Streptomyces spore concentrations, the concentration ratios of Streptomyces spores to pathogen spores, and inoculation methods. The results suggested that wheat sprouts harbored diverse endophytic Streptomyces species which derived from wheat seeds, these strains should be more likely transmitted to the next generation, and confer competitive ability to pathogens on the offspring. Owing to the more intimate correlation between sprout endophytic flora with host plants, these strains are more suitable for mature plant interiors compared with those from rhizosphere soils and root interiors.

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Funding

This work was supported by grants from Chinese National Natural Science Foundation (Grant No.: 31700288), Chinese National Natural Science Foundation (Grant No.: 31971384) and Youth Elite Project of Guangzhou University of Chinese Medicine (Grant No.: QNYC20170103).

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Author notes

  1. Shunsheng Deng and Yanming Liu are co-first authors.

  2. Shunsheng Deng and Yanming Liu contributed equally to this work.

Authors and Affiliations

  1. College of Fundamental Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, People’s Republic of China

    Shunsheng Deng & Yali Huang

  2. School of Basic Courses, Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of China

    Yanming Liu & Zujun Deng

Authors
  1. Shunsheng Deng
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  2. Yanming Liu
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  3. Zujun Deng
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  4. Yali Huang
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Contributions

YH and ZD designed research and wrote the paper. SD and YL performed research and analyzed data.

Corresponding authors

Correspondence to Zujun Deng or Yali Huang.

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Communicated by Erko Stackebrandt.

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Deng, S., Liu, Y., Deng, Z. et al. Isolation of actinobacterial endophytes from wheat sprouts as biocontrol agents to control seed pathogenic fungi. Arch Microbiol 203, 6163–6171 (2021). https://doi.org/10.1007/s00203-021-02581-3

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  • DOI: https://doi.org/10.1007/s00203-021-02581-3

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