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Salt tolerance of halotolerant bacteria from coastal soils and sediments near saltern field of Hainan Island, China

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Abstract

Understanding the salt tolerance of microbial communities may help to elucidate the effects of salt concentration and other environmental factors on soil biodiversity. Here, high-throughput sequencing of 16S rDNA and ITS was combined to investigate the distribution and salt tolerance of microbial communities in coastal soils and sediments near the Yinggehai saltern field of Hainan Island, China. The microbial communities in the soils and sediments of the land zone (YGHLS), the intertidal zone (YGHIS), and the inshore zone (YGHWS) were compared. PCoA of weighted and unweighted UniFrac distance revealed obvious differences in soil microbial community among different samples. ANOSIM analysis could clearly separate the three samples from each other. Three halotolerant bacteria, including Halomonas, Halobacillus and Wallemia, were found in the samples, which accounted for 0.0335 ± 0.0586%, 0.0241 ± 0.0304%, and 0.0308 ± 0.0445% of the total microbial community, respectively. The relative abundance of Trk system potassium uptake protein, Kdp operon response regulator, and Na+/H+ antiporter in the samples exceeded 0.09%, 0.06%, and 0.02%, respectively, indicating that the Trk system plays a major role in the salt tolerance of halotolerant bacteria in Yinggehai coastal soils and sediments.

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Acknowledgements

This work was supported by Key Research and Development Projects of Hainan Province (ZDYF2019212), Natural Science Foundation of Hainan Province (318MS072) and Projects of Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources, Ministry of Education (UCTMB20205).

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Authors and Affiliations

  1. Key Laboratory of Utilization and Conservation for Tropical Marine Bio resources (Hainan Tropical Ocean University), Ministry of Education, Sanya, 572022, China

    Yonggan Chen

  2. Hainan Key Laboratory for Conservation and Utilization of Tropical Marine Fishery Resources, Hainan Tropical Ocean University, Sanya, 572022, China

    Yonggan Chen & Hongwei Luo

  3. Key Laboratory of Biosafety, Nanjing Institute of Environmental Sciences, Nanjing, 210042, China

    Zhenhua Zhang

  4. College of Fisheries and Life Science, Hainan Tropical Ocean University, Sanya, 572022, China

    Yonggan Chen & Zhen Li

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  1. Yonggan Chen
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  2. Zhenhua Zhang
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  3. Hongwei Luo
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  4. Zhen Li
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Correspondence to Zhenhua Zhang.

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Chen, Y., Zhang, Z., Luo, H. et al. Salt tolerance of halotolerant bacteria from coastal soils and sediments near saltern field of Hainan Island, China. Arch Microbiol 203, 5921–5930 (2021). https://doi.org/10.1007/s00203-021-02461-w

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  • DOI: https://doi.org/10.1007/s00203-021-02461-w

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