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Arthrobacter sulfonylureivorans sp. nov., isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil

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Abstract

A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil. The optimal temperature and pH for the growth of strain LAM7117T were 35 °C and 7.5, respectively. Strain LAM7117T could grow in the presence of NaCl with concentration up to 9% (w/v). Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%). The values of digital DNA–DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively. The genomic DNA G + C content was 65.9 mol%. The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid. The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid. The predominant menaquinones of strain LAM7117T were MK-8 and MK-9. Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp. nov. is proposed. The type strain is LAM7117T (= JCM 32824T = CGMCC 1.16681T).

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Abbreviations

JCM:

Japan collection of microorganisms

ANI:

Average nucleotide identity

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Acknowledgements

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain LAM7117T is MG009252. The GenBank accession number for the draft genome sequence of strain LAM7117T is RJAE01000000.

Funding

This work was supported by National Natural Science Foundation of China (NSFC, No. 31670006). This work was also supported by the National Key R&D Program of China (2017YFD0800702) and the Fundamental Research Funds for the Chinese Academy of Agricultural Sciences (Y2020GH14).

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Author notes

  1. Xiaoyan Han, Qi Zhang and Qingyun Ma contributed equally to this work.

Authors and Affiliations

  1. Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People’s Republic of China

    Xiaoyan Han, Qingyun Ma, Delong Kong, Yiqing Zhou, Xu Jiang & Zhiyong Ruan

  2. College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, 330045, People’s Republic of China

    Xiaoyan Han

  3. College of Life Science, Xinjiang Normal University, Urumqi, 830046, People’s Republic of China

    Qi Zhang & Wei Zhang

  4. College of Resources and Environment, Tibet Agricultural and Animal Husbandry University, Linzhi, 860000, People’s Republic of China

    Zhiyong Ruan

  5. CAAS-CIAT Joint Laboratory in Advanced Technologies for Sustainable Agriculture, Beijing, 00081, People’s Republic of China

    Xu Jiang

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  1. Xiaoyan Han
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Han, X., Zhang, Q., Ma, Q. et al. Arthrobacter sulfonylureivorans sp. nov., isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil. Arch Microbiol 203, 1039–1045 (2021). https://doi.org/10.1007/s00203-020-02097-2

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