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Synergistic deletion of RGS1 and COS1 may reduce the pathogenicity of Magnaporthe oryzae

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Abstract

Rice blast, caused by Magnaporthe oryzae, is a serious threat to global rice production. In recent years, many pathogenicity genes of M. oryzae have been identified, although most of their functions remain unknown. In this study, we report the synergistic deletion of RGS1 and COS1 that may reduce the pathogenicity of M. oryzae. The investigation involved comparing ΔMorgs1, ΔMocos1, and ΔMorgs1/ΔMocos1 mutants. The ΔMorgs1/ΔMocos1 mutant showed a weak reduction in vegetative growth, and the colonies displayed fewer and smoother aerial hyphae. The ΔMorgs1/ΔMocos1 mutant exhibited delayed appressorium-like structure formation and ‘low pathogenicity’ on detached rice seedling leaves when compared with ΔMocos1. Moreover, the melanin content of the single and double mutants was remarkably lower than that of the WT type. Thus, our results indicate that the synergy between RGS1 and COS1 may be crucial in the pathogenicity of M. oryzae.

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Abbreviations

CM:

Complete medium

DHN:

1,8-Dihydroxynaphthalene

OMA:

Oatmeal agar

qRT-PCR:

Quantitative reverse-transcription-polymerase chain reaction

WT:

Wild type

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Funding

This work was supported by the National Natural Science Foundation of China (Grant no. 31260418).

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Authors and Affiliations

  1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China

    He Na, An Bang, Xie Qing-biao, Yan Xia, Feng Hui-min, Luo Hong-li & He Chao-zu

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  1. He Na
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  2. An Bang
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  3. Xie Qing-biao
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  4. Yan Xia
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  5. Feng Hui-min
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  6. Luo Hong-li
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  7. He Chao-zu
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Correspondence to He Chao-zu.

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Communicated by Erko Stackebrandt.

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Na, H., Bang, A., Qing-biao, X. et al. Synergistic deletion of RGS1 and COS1 may reduce the pathogenicity of Magnaporthe oryzae. Arch Microbiol 201, 807–816 (2019). https://doi.org/10.1007/s00203-019-01646-8

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  • DOI: https://doi.org/10.1007/s00203-019-01646-8

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