Abstract
A bacterial strain, designated GM-8T, which was isolated from seawater around Pohang in Republic of Korea, was subjected to a polyphasic taxonomic study. It was lipolytic, Gram-stain-negative, aerobic, non-motile and coccoid, ovoid, or rod-shaped. Strain GM-8T grew optimally at 30 °C and in the presence of 2.0–3.0% (w/v) NaCl. The phylogenetic trees of 16S rRNA gene sequences based on three algorithms showed that strain GM-8T joined the clade comprising the type strains of Zhongshania species. The novel strain exhibited the highest 16S rRNA gene sequence similarity value (98.6%) to Zhongshania borealis CL-AS9T and sequence similarities of 97.7–98.3% to the type strains of three other Zhongshania species. Strain GM-8T contained Q-8 as the predominant ubiquinone and C17:1 ω8c, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and C18:1 ω7c as the major fatty acids. Phosphatidylglycerol and phosphatidylethanolamine were major polar lipids found in strain GM-8T and the type strain of Zhongshania antarctica. The DNA G + C content of strain GM-8T was 50.9 mol%. Mean DNA–DNA relatedness values between strain GM-8T and the type strains of four other Zhongshania species were 13.3–20.3%. Its differential phenotypic traits, together with the phylogenetic and genetic evidences, revealed that strain GM-8T is distinct from recognized species of the genus Zhongshania. On the basis of the data presented, strain GM-8T represents a novel species of the genus Zhongshania, for which the name Zhongshania ponticola sp. nov. is proposed. The type strain is GM-8T (= KCTC 62425T = KACC 19616T = NBRC 113193T).
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Acknowledgements
This work was supported by the project on survey of indigenous species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) and by a Grant from the KRIBB Research Initiative Program of the Republic of Korea. We are grateful to Professor Che Ok Jeon of Chung-Ang University for kindly providing Z. aliphaticivorans SM-2T.
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Communicated by Erko Stackebrandt.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain W-GM-8T is MG816569. DPD (digital protologue database) Taxon number: TA00450.
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Park, S., Park, JM., Lee, JS. et al. Zhongshania ponticola sp. nov., a novel lipolytic bacterium of the class Gammaproteobacteria isolated from seawater. Arch Microbiol 200, 1177–1182 (2018). https://doi.org/10.1007/s00203-018-1538-7
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DOI: https://doi.org/10.1007/s00203-018-1538-7