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Pseudoruegeria litorisediminis sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from a tidal flat

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Abstract

A lipolytic bacterial strain, designated BPTF-M5T, was isolated from a tidal flat sediment of the Yellow Sea in Republic of Korea, and identified by a polyphasic taxonomic approach. It was Gram negative, aerobic, non-motile and rod shaped. Strain BPTF-M5T grew optimally at 30 °C and in the presence of 2.0–3.0% (w/v) NaCl. A neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BPTF-M5T fell within the clade comprising the type strains of Pseudoruegeria species. The novel strain exhibited the highest 16S rRNA gene sequence similarity value (98.6%) to Pseudoruegeria marinistellae SF-16T. Strain BPTF-M5T exhibited sequence similarities of 97.0–98.5% to the type strains of five other Pseudoruegeria species and of less than 96.1% to other recognized species. Strain BPTF-M5T contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the major fatty acid. Phosphatidylglycerol, one unidentified aminolipid, one unidentified lipid and one unidentified glycolipid were detected as major polar lipids in the novel strain. The DNA G + C content of strain BPTF-M5T was 63.3 mol%. DNA–DNA relatedness values between strain BPTF-M5T and the type strains of the six Pseudoruegeria species were 11.0–24.3%. The phenotypic properties of strain BPTF-M5T were clearly distinguished from those of the type strains of the six Pseudoruegeria species. Based on the polyphasic data presented, strain BPTF-M5T represents a novel species of the genus Pseudoruegeria, for which the name Pseudoruegeria litorisediminis sp. nov. is proposed. The type strain is BPTF-M5T (= KCTC 62420T = KACC 19613T = NBRC 113189T).

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Acknowledgements

This work was supported by the project on survey of indigenous species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) and by a grant from the KRIBB Research Initiative Program of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea

    Sooyeon Park, Ji-Min Park & Jung-Hoon Yoon

  2. Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeongeup, Republic of Korea

    Jung-Sook Lee

  3. Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Daejeon, Republic of Korea

    Tae-Kwnag Oh

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  1. Sooyeon Park
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  2. Ji-Min Park
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  3. Jung-Sook Lee
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  4. Tae-Kwnag Oh
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  5. Jung-Hoon Yoon
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Corresponding author

Correspondence to Jung-Hoon Yoon.

Additional information

Communicated by Erko Stackebrandt.

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain BPTF-M5T is MH143794. Digital protologue’ database (DPD) Taxon number: TA00474.

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Park, S., Park, JM., Lee, JS. et al. Pseudoruegeria litorisediminis sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from a tidal flat. Arch Microbiol 200, 1183–1189 (2018). https://doi.org/10.1007/s00203-018-1539-6

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  • DOI: https://doi.org/10.1007/s00203-018-1539-6

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