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Study of the correlation between fengycin promoter expression and its production by Bacillus subtilis under different culture conditions and the impact on surfactin production

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Abstract

This work aimed to rely expression of the fengycin promoter to fengycin production under different culture conditions. To this end, Bacillus subtilis BBG208, derived from BBG21, which is a fengycin overproducing strain carrying the green fluorescent protein (GFP) under the control of fengycin promoter, was used to assess the effects of different carbon and nitrogen sources on surfactin and fengycin production and the fengycin promoter expression. The data showed that some carbon sources oriented synthesis of one family of lipopeptides, while most of the nitrogen sources allowed high co-production of fengycin and surfactin. High expressions of promoter Pfen and fengycin synthesis were obtained with urea or urea + ammonium mixture as nitrogen source and mannitol as carbon source. Moreover, temperature, pH and oxygenation influenced their biosynthesis based on the nutrition conditions. Optimization of the production medium increased the fengycin production to 768 mg L−1, which is the highest level reported for this strain. This study defines the suitable nutrient conditions allowing as well the highest expression of the fengycin promoter and portrays the conditions relying on the fengycin and surfactin production.

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Acknowledgements

Yazen Yaseen received a PhD grant from the Iraqi and French governments through Campus France. We thank our colleagues from the REALCAT platform for their collaboration. This platform benefited from a government subvention administered by the French National Research Agency (ANR) with the contractual reference ANR-11-EQPX-0037.The authors also thank the CPER/FEDER Alibiotech obtained from la region des Hauts-de-France.

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  1. Charles Viollette Institute, University Lille 1, Sciences and Technologies, Cité Scientifique, 59655, Villeneuve d’Ascq, France

    Yazen Yaseen, Frédérique Gancel, Max Béchet, Djamel Drider & Philippe Jacques

  2. Terra Teaching and Research Centre, Microbial Processes and Interactions, Gembloux Agro-Bio Tech, University of Liege, Gembloux, B-5030, Liège, Belgium

    Yazen Yaseen, Frédérique Gancel, Max Béchet, Djamel Drider & Philippe Jacques

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  1. Yazen Yaseen
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  2. Frédérique Gancel
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Correspondence to Philippe Jacques.

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Communicated by Erko Stackebrandt.

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Yaseen, Y., Gancel, F., Béchet, M. et al. Study of the correlation between fengycin promoter expression and its production by Bacillus subtilis under different culture conditions and the impact on surfactin production. Arch Microbiol 199, 1371–1382 (2017). https://doi.org/10.1007/s00203-017-1406-x

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