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Two new species of the genus Streptomyces: Streptomyces camponoti sp. nov. and Streptomyces cuticulae sp. nov. isolated from the cuticle of Camponotus japonicus Mayr

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Abstract

Two novel actinomycetes, designated strains 2C-SSA16(2)T and 1C-GS8T, were isolated from the cuticle of Camponotus japonicus Mayr, collected from Northeast Agricultural University, Heilongjiang Province, north China. Both of them contained genes (involved in antibiotics biosynthesis) of the ketosynthase (KS) and methyl malonyl transferase domains (PKS-I) and the adenylation domain (NRPS). A polyphasic study was carried out to establish the taxonomic positions of these strains. The 16S rRNA gene sequence analysis showed that the two novel isolates 2C-SSA16(2)T and 1C-GS8T exhibited 98.8% similarity with each other and that they are most closely related to Streptomyces umbrinus JCM 4521T (99.0, 98.6%), Streptomyces ederensis JCM 4958T (98.9, 98.7%), Streptomyces aurantiacus JCM 4453T (98.6, 98.2%), Streptomyces glomeroaurantiacus JCM 4677T (98.6, 98.1%), Streptomyces tauricus JCM4837T (98.2, 98.0%) and Streptomyces phaeochromogenes JCM 4070T (98.2, 99.2%). The corresponding phylogenetic analysis based on partial gyrB gene sequences showed that strains 2C-SSA16(2)T and 1C-GS8T formed a cluster with the above-mentioned strains. The DNA–DNA hybridization data and phenotypic characteristics indicated that strains 2C-SSA16(2)T and 1C-GS8T could be readily distinguished from each other and their closest phylogenetic relatives. Therefore, these two strains are suggested to represent two novel species of the genus Streptomyces, for which the names Streptomyces camponoti sp. nov. and Streptomyces cuticulae sp. nov. are proposed. The type strains are 2C-SSA16(2)T (=CGMCC 4.7276T = DSM 100522T) and 1C-GS8T (=CGMCC 4.7348 = DSM 103127T), respectively.

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Acknowledgements

This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Key Technology R&D Program (No. 2012BAD19B06), the National Natural Science Foundation of China (No. 31471832, 31171913, 31500010, 31572070 and 31372006), Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP) and the “Young Talents” Project of Northeast Agricultural University (14QC02).We are grateful to Prof. Aharon Oren for helpful advice on the specific epithet.

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    1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

      Chenyu Piao, Weiwei Zheng, Yao Li, Chongxi Liu, Liying Jin, Wei Song, Kai Yan, Xiangjing Wang & Wensheng Xiang

    2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

      Wensheng Xiang

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    Communicated by Erko Stackebrandt.

    C. Piao and W. Zheng contributed equally to this work.

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    Piao, C., Zheng, W., Li, Y. et al. Two new species of the genus Streptomyces: Streptomyces camponoti sp. nov. and Streptomyces cuticulae sp. nov. isolated from the cuticle of Camponotus japonicus Mayr. Arch Microbiol 199, 963–970 (2017). https://doi.org/10.1007/s00203-017-1353-6

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