Abstract
Enterococcus sp. 812, isolated from fresh broccoli, was previously found to produce a bacteriocin active against a number of Gram-positive bacteria, including Listeria monocytogenes. Bacteriocin activity decreased slightly after autoclaving (121 °C for 15 min), but was inactivated by protease K. Mass spectrometry analysis revealed the bacteriocin mass to be approximately 4,521.34 Da. N-terminal amino acid sequencing yielded a partial sequence, NH2-ATYYGNGVYXDKKKXWVEWGQA, by Edman degradation, which contained the consensus class IIa bacteriocin motif YGNGV in the N-terminal region. The obtained partial sequence showed high homology with some enterococcal bacteriocins; however, no identical peptide or protein was found. This peptide was therefore considered to be a novel bacteriocin produced by Enterococcus sp. 812 and was termed enterocin T.
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Acknowledgments
The authors would like to thank the National Science Council Taiwan for financially supporting this study through a grant to Shwu-fen Pan (Contract No. NSC 101-2313-B-130-001).
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Communicated by Djamel Drider.
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Chen, Ys., Yu, Cr., Ji, Sh. et al. Enterocin T, a novel class IIa bacteriocin produced by Enterococcus sp. 812. Arch Microbiol 195, 655–660 (2013). https://doi.org/10.1007/s00203-013-0917-3
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DOI: https://doi.org/10.1007/s00203-013-0917-3