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Characterization of Deinococcus sahariens sp. nov., a radiation-resistant bacterium isolated from a Saharan hot spring

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Abstract

An ultraviolet-radiation-resistant, Gram-positive, orange-pigmented, thermophilic and strictly aerobic cocci was isolated from Saharan water hot spring in Tunisia. The newly isolated bacterium, designated HAN-23T, was identified based on polyphasic taxonomy including genotypic, phenotypic and chemotaxonomic characterization. Phylogenetic analysis based on 16S rRNA gene sequences placed this strain within Deinococcus genus. However, strain HAN-23T is different from recognized species of the genus Deinococcus, showing less than 94.0% similarity values to its closest relatives. The predominant cellular fatty acids determined by gas chromatography were iso-C15:0, iso-C17:0 and iso C17:1 ω9c. The major respiratory quinone was MK-8. The DNA G + C content was 66.9 mol%. DNA–DNA hybridization measurements revealed low DNA relatedness (6%) between the novel isolate and its closest neighbor, the type strain Deinococcus geothermalis DSM 11300. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain HAN-23T represents a novel species of the genus Deinococcus, for which the name Deinococcus sahariens sp. nov. is proposed, the type strain being HAN-23T (=DSM 18496T; LMG 23756T).

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Acknowledgments

This work was supported by the Ministry of Higher Education and Scientific Research in Tunisia and the French Tunisian Mixed Committee of University Cooperation (CMCU Projects).

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Authors and Affiliations

  1. Unité de Biochimie et Biologie Moléculaire, Université de Tunis El Manar, Campus Universitaire, 2092, Tunis, Tunisia

    Hanene Bouraoui, Melek Ben Aissa & Mohamed Manai

  2. Peptidome de la Peau des Amphibiens FRE 2852- UPMC/CNRS, Université Pierre et Marie Curie, Paris, France

    Feten Abbassi

  3. INRA, UMR FARE-URCA 614, 8 Rue Gabriel-Voisin, BP 316, Reims, France

    Jean Pierre Touzel

  4. Université de Toulouse, INSA, LISBP, 135 Avenue de Rangueil, 31077, Toulouse, France

    Michael O’donohue

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  1. Hanene Bouraoui
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  2. Melek Ben Aissa
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  4. Jean Pierre Touzel
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  6. Mohamed Manai
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Correspondence to Hanene Bouraoui.

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Communicated by Erko Stackebrandt.

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Bouraoui, H., Aissa, M.B., Abbassi, F. et al. Characterization of Deinococcus sahariens sp. nov., a radiation-resistant bacterium isolated from a Saharan hot spring. Arch Microbiol 194, 315–322 (2012). https://doi.org/10.1007/s00203-011-0762-1

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