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Genetic diversity of rhizobia associated with Vicia faba in three ecological regions of China

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Abstract

Great genetic diversity was revealed among 75 rhizobal isolates associated with Vicia faba grown in Chinese fields with AFLP, ARDRA, 16S rDNA sequencing, DNA–DNA hybridization, BOX-PCR and RFLP of PCR-amplified nodD and nodC. Most of the isolates were Rhizobium leguminosarum, and six isolates belonged to an unnamed Rhizobium species. In the homogeneity analysis, the isolates were grouped into three clusters corresponding to (1) autumn sowing (subtropical) region where the winter ecotype of V. faba was cultivated, (2) spring sowing (temperate) region where the spring ecotype was grown, and (3) Yunnan province where the intermediate ecotype was sown either in spring or in autumn. Nonrandom associations were found among the nod genotypes, genomic types and ecological regions, indicating an epidemic symbiotic gene transfer pattern among different genomic backgrounds within an ecological region and a relatively limited transfer pattern between different regions. Conclusively, the present results suggested an endemic population structure of V. faba rhizobia in Chinese fields and demonstrated a novel rhizobium associated with faba bean.

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Acknowledgments

We thank Dr. Min Qi Lu, Dr. Qiang Chen and other collaborators for their valuable contribution in collecting and isolating the rhizobia. This work was financial supported by the foundation of the State Key Basic Research and Development Plan of China (grant 2006CB100206), and by National Project for Basic S&T Platform Construction (grant 2005DKA21201-10).

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Authors and Affiliations

  1. Key laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, 100094, Beijing, China

    Chang Fu Tian, En Tao Wang, Tian Xu Han, Xin Hua Sui & Wen Xin Chen

  2. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340,  México D.F., México

    En Tao Wang

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  1. Chang Fu Tian
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  2. En Tao Wang
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  3. Tian Xu Han
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Correspondence to Wen Xin Chen.

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Communicated by Erko Stackebrandt.

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Tian, C.F., Wang, E.T., Han, T.X. et al. Genetic diversity of rhizobia associated with Vicia faba in three ecological regions of China. Arch Microbiol 188, 273–282 (2007). https://doi.org/10.1007/s00203-007-0245-6

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  • DOI: https://doi.org/10.1007/s00203-007-0245-6

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