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Physiological, biochemical, and genetic characterization of an alicyclic amine-degrading Mycobacterium sp. strain THO100 isolated from a morpholine-containing culture of activated sewage sludge

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Abstract

Mycobacterium sp. strain THO100 was isolated from a morpholine-containing culture of activated sewage sludge. This strain was able to utilize pyrrolidine, morpholine, piperidine, piperazine, and 1,2,3,6-tetrahydropyridine as the sole sources of carbon, nitrogen, and energy. The degradation pathway of pyrrolidine as the best substrate for cellular growth was proposed based on the assays of substrate-induced cytochrome P450 and constitutive enzyme activities toward 4-aminobutyric acid (GABA) and succinic semialdehyde (SSA). Its 16S ribosomal RNA gene sequence (16S rDNA) was identical to that of Mycobacterium tokaiense ATCC 27282T. The morABC genes responsible for alicyclic amine degradation were nearly identical among different species of Mycobacteria. Remarkably, repetitive sequences at the intergenic spacer (IGS) region between morC and orf1’ were detected by comparison of the nearly identical mor gene cluster regions. Considering the strain activity for alicyclic amine degradation, the deleted 65-bp DNA segment did not significantly alter the open reading frames, and the expression and functions of the P450mor system remained unaltered. In addition, we found a spontaneous deletion of P450mor from another strain HE5 containing the archetypal mor gene cluster, which indicated a possible occurrence of DNA recombination to rearrange the DNA.

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Acknowledgments

This study was supported partly by a fellowship program from the Deutscher Akademischer Austauschdienst (DAAD) and by the BK21 research program from the Ministry of Education and Human Resources Development, Republic of Korea.

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Authors and Affiliations

  1. School of Biological Sciences, Seoul National University, San 56-1 Shinrim, Kwanak, Seoul, Republic of Korea

    Yong-Hak Kim, Ilnam Kang & Sang-Jong Kim

  2. Bioconversion and Sustainable Development, Biotechnology Research Institute, NRC Canada, 6100 Royalmount Avenue, H4P 2R2, Montreal, QC, Canada

    Hélène Bergeron & Peter C. K. Lau

  3. Abteilung Biologische Abluftreinigung, ISWA, Universität Stuttgart, Bandtäle 2, Stuttgart, Germany

    Karl-Heinrich Engesser

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  1. Yong-Hak Kim
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  2. Ilnam Kang
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  3. Hélène Bergeron
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  4. Peter C. K. Lau
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  5. Karl-Heinrich Engesser
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  6. Sang-Jong Kim
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Correspondence to Sang-Jong Kim.

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Kim, YH., Kang, I., Bergeron, H. et al. Physiological, biochemical, and genetic characterization of an alicyclic amine-degrading Mycobacterium sp. strain THO100 isolated from a morpholine-containing culture of activated sewage sludge. Arch Microbiol 186, 425–434 (2006). https://doi.org/10.1007/s00203-006-0157-x

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  • DOI: https://doi.org/10.1007/s00203-006-0157-x

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