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Effect of SsrA (tmRNA) tagging system on translational regulation in Streptomyces

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Abstract

ssrA genes encoding tmRNA with transfer and messenger RNA functions are ubiquitous in bacteria. In a process called trans-translation, tmRNA enters a stalled ribosome and allows release of the original mRNA, then tmRNA becomes the template for translation of a short tag that signals for proteolytic degradation. We provide here the first evidences that the tmRNA tagging system (ssrA and cohort smpB) is active in Streptomyces. Transcription of the genes was shown and construction of a genetic probe allowed detection of a tmRNA-tagged peptide. Obtention of ssrA and smpB mutants of Streptomyces lividans showed that the ssrA system is dispensable in Streptomyces. Morphologies of the mutants colonies were similar to the wild type, thus tmRNA-mediated tagging does not seem to have, under conditions used, a significant effect in the Streptomyces differentiation.

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Acknowledgements

We thank J. Viala for her advice and constant interest throughout this study and Alex Edelman & Associates for correcting this manuscript. We are grateful to J.M. Ghigo for his gift of plasmid pKOBEG and advices and to Philippe Bouloc for providing E. coli strain 1512(p10Sa-H7). This research was supported by the Pasteur Institute, the Centre National de la Recherche Scientifique, a grant from Rhône Poulenc Rorer, the Université Paris 7, and QLK3-CT2000-00122 from the European Commission.

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Author notes

  1. Celine Lavire

    Present address: CL, UMR CNRS 5557 Microbial Ecology, Université Claude Bernard, Lyon 1, 69622, Villeurbanne, France

  2. Audrey Bellier

    Present address: AB, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, CA, 92093-0349, USA

Authors and Affiliations

  1. UP Génétique Bactérienne et Différenciation, Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France

    Sandrine Braud, Celine Lavire, Audrey Bellier & Philippe Mazodier

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  1. Sandrine Braud
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  2. Celine Lavire
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  4. Philippe Mazodier
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Correspondence to Philippe Mazodier.

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Braud, S., Lavire, C., Bellier, A. et al. Effect of SsrA (tmRNA) tagging system on translational regulation in Streptomyces . Arch Microbiol 184, 343–352 (2006). https://doi.org/10.1007/s00203-005-0051-y

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  • DOI: https://doi.org/10.1007/s00203-005-0051-y

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