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Chlorinated aliphatic hydrocarbon-induced degradation of trichloroethylene in Wautersia numadzuensis sp. nov.

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Abstract

Two strains of trichloroethylene (TCE)-degrading bacteria were isolated from soils at polluted and unpolluted sites. The isolates, strains TE26T and K6, showed co-substrate-independent TCE-degrading activity. TCE degradation was accelerated by preincubation with tetrachloroethylene, cis-dichloroethylene (DCE) and 1,1-DCE. TCE-degrading activities of strains TE26T and K6 were 0.23, 0.24 μmol min−1 g−1 dry cells, respectively. 16S rDNA sequences of strains TE26T and K6 were almost identical (99.7% similarity), and most closely related to Ralstonia basilensis (ATCC17697T) (98.5% similarity). From the results of DNA–DNA hybridizations, strain TE26T was genetically coherent to strain K6 (94 and 88% hybridization), and exhibited lower relatedness to R. basilensis (DSM11853T) (44% and 15%). In addition, because of the differences in chemotaxonomic properties, strain TE26T and strain K6 appear to be distinct from all established species of the Ralstonia group. Based on these results and the proposal of transferring R. basilensis and related species to Wautersia gen. nov., we propose that these strains should be assigned to the genus Wautersia as Wautersia numadzuensis sp. nov.

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Abbreviations

DCE:

Dichloroethylene

LB:

Luria-Bertani

OD:

Optical density

PCE:

Tetrachloroethylene

TCE:

Trichloroethylene

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Acknowledgements

This study was supported by a research grant from Shizuoka Prefecture. We thank Prof. T. Ohmori and Dr H. Nojiri (Tokyo University) for pertinent advice at the beginning of this work, and Prof. A. Hiraishi and Dr H. Futamata (Toyohashi University of Technology) for helpful advice on this work and manuscript, and Dr M. Nishijima (NCIMB Japan) for offering electron micrograph techniques.

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Authors and Affiliations

  1. Numazu Industrial Research Institute of Shizuoka Prefecture, Ohoka, Numazu, Shizuoka, 410-0022, Japan

    Chizuko Kageyama, Toshiya Ohta, Kazuyo Hiraoka, Morimasa Suzuki, Tetsuji Okamoto & Kazuo Ohishi

  2. Shizuoka Agricultural experiment station, 678-1 Tomioka, Toyoda-cho, Iwata-gun, Shizuoka, 438-0803, Japan

    Chizuko Kageyama

  3. Shizuoka Industrial Research Institute of Shizuoka Prefecture, 2078, Makigaya, Shizuoka-shi, 421-1298, Japan

    Kazuyo Hiraoka & Tetsuji Okamoto

  4. Living Environment Office, Shizuoka Prefectural Government, 9-6 Otemachi, Shizuoka City, 420-8601, Japan

    Morimasa Suzuki

Authors
  1. Chizuko Kageyama
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  2. Toshiya Ohta
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  3. Kazuyo Hiraoka
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  4. Morimasa Suzuki
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  5. Tetsuji Okamoto
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  6. Kazuo Ohishi
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Correspondence to Kazuo Ohishi.

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Kageyama, C., Ohta, T., Hiraoka, K. et al. Chlorinated aliphatic hydrocarbon-induced degradation of trichloroethylene in Wautersia numadzuensis sp. nov.. Arch Microbiol 183, 56–65 (2005). https://doi.org/10.1007/s00203-004-0746-5

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  • DOI: https://doi.org/10.1007/s00203-004-0746-5

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