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Cloning and partial characterization of the gene encoding the putative elongation factor Ts of Streptococcus suis serotype 2

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Abstract

Streptococcus suis infection has a substantial impact on the swine industry. In addition, S. suis serotype 2 is recognized as a zoonotic agent. In this paper, we report the cloning and complete sequence of the gene coding for the putative elongation factor Ts (tsf-like) of S. suis. The putative tsf gene seems to be transcribed from a promoter located within the cloned DNA fragment, as its expression is not dependent on insertional orientation within the plasmid. One copy of the tsf gene was detected in the chromosome of S. suis by Southern blot analysis. Interestingly, the elongation factor Ts expressed by all reference strains of all S. suis serotypes were antigenically similar, as determined by Western blot.

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References

  • Allgaier A, Goethe R, Wisselink HJ, Smith HE, Valentin-Weigand P (2001) Relatedness of Streptococcus suis isolates of various serotypes and clinical background as evaluated by macrorestriction analysis and expression of potential virulence traits. J Clin Microbiol 39:445–453

    CAS  PubMed  Google Scholar 

  • An G, Bendiak DS, Mamelak LA, Friesen JD (1981) Organization and nucleotide sequence of a new ribosomal operon in Escherichia coli containing the genes for ribosomal protein S2 and elongation factor Ts. Nucleic Acids Res 9:4163–4172

    CAS  PubMed  Google Scholar 

  • Arends JP, Zanen HC (1988) Meningitis caused by Streptococcus suis in humans. J Infect Dis 10:131–137

    CAS  Google Scholar 

  • Bendiak DS, Friesen JD (1981) Organization of genes in the four minute region of the Escherichia coli chromosome: evidence that rpsB and tsf are co-transcribed. Mol Gen Genet 181:356–362

    CAS  PubMed  Google Scholar 

  • Berthelot-Herault F, Marois C, Gottschalk M, Kobich M (2002) Genetic diversity of Streptococcus suis strains isolated from pigs and humans as revealed by pulsed-field gel electrophoresis. J Clin Microbiol 40:615–619

    Article  CAS  PubMed  Google Scholar 

  • Blattner FR, et al (1997) The complete genome sequence of Escherichia coli K-12. Science 277:1453–1474

    CAS  PubMed  Google Scholar 

  • Bolotin A, et al (2001) The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403. Genome Res 11:731–753

    CAS  PubMed  Google Scholar 

  • Campo Sepulveda EM del, Altman E, Kobisch M, D′Allaire S, Gottschalk M (1996) Detection of antibodies against Streptococcus suis capsular type 2 using a purified capsular polysaccharide antigen-based indirect ELISA. Vet Microbiol 52:113–125

    Article  PubMed  Google Scholar 

  • Charland N, Jacques M, Lacouture S, Gottschalk M (1997) Characterization and protective activity of a monoclonal antibody against a capsular epitope shared by Streptococcus suis serotypes 1, 2 and 1/2. Microbiology 143:3607–3614

    CAS  PubMed  Google Scholar 

  • Chatellier S, Gottschalk M, Higgins R, Brousseau R, Harel J (1999) Relatedness of Streptococcus suis serotype 2 isolates from different geographic origins as evaluated by molecular fingerprinting and phenotyping. J Clin Microbiol 37:362–366

    CAS  PubMed  Google Scholar 

  • Ferretti JJ, Curtis R III (1987) Compilation of nucleotide sequences that signal the initiation of transcription and translation in streptococci. In: American Society for Microbiology (ed) Streptococcal genetics. American Society for Microbiology, Washington, D.C., p. 293

  • Gherna R, Pienta P, Cote R (1992) Catalogue of bacteria and phages. American Type Culture Collection, Rockville, Md.

  • Gottschalk M, Lebrun A, Wisselink HJ, Dubreuil JD, Smith HE, Vetch U (1998) Production of virulence-related proteins by Canadian strains of Streptococcus suis capsular type 2. Can J Vet Res 62:75–79

    CAS  PubMed  Google Scholar 

  • Higgins R, Gottschalk M (1999) Streptococcal disease. In: Straw B, D′Allaire S, Mengeling W, Taylor D (eds) Diseases of swine, 8th edn. Iowa State University Press, Ames, pp 563–578

  • Higgins R, Gottschalk M (2001) Distribution of Streptococcus suis capsular types in 2000. Can Vet J 42:223

    CAS  PubMed  Google Scholar 

  • Hofmann K, Bucher P (1996) The UBA domain: a sequence motif present in multiple enzyme classes of the ubiquitination pathway. Trends Biochem Sci 21:172–173

    CAS  PubMed  Google Scholar 

  • Hwang YW, Sanchez A, Miller DL (1989) Mutagenesis of bacterial elongation factor Tu at lysine 136. A conserved amino acid in GTP regulatory proteins. J Biol Chem 264:8304–8309

    CAS  PubMed  Google Scholar 

  • Le Dantec L, Bove JM, Saillard C (1998) Gene organization and transcriptional analysis of the Spiroplasma citri rpsB/tsf/x operon. Curr Microbiol 37:269–273

    Article  PubMed  Google Scholar 

  • Lewin B (2000) Protein synthesis. In: Oxford University Press (ed) Genes VII, 7th edn. Oxford University Press, New York

  • Makarova KS, et al (1999) Comparative genomics of the Archaea (Euryarchaeota): evolution of conserved protein families, the stable core, and the variable shell. Genome Res 9:608–628

    CAS  PubMed  Google Scholar 

  • Ohnishi M, et al (1999) Molecular cloning, sequencing, purification, and characterization of Pseudomonas aeruginosa ribosome recycling factor. J Bacteriol 181:1281–1291

    CAS  PubMed  Google Scholar 

  • Overweg K, et al (2000) Differential protein expression in phenotypic variants of Streptococcus pneumoniae. Infect Immun 68:4604–4610

    CAS  PubMed  Google Scholar 

  • Quessy S, Dubreuil JD, Caya M, Higgins R (1995) Discrimination of virulent and avirulent Streptococcus suis capsular type 2 isolates from different geographical origins. Infect Immun 63:1975–1979

    CAS  PubMed  Google Scholar 

  • Rice PM, Elliston K, Gribskov M (1992) DNA. In: Gribskov M, Devereaux J (eds) Sequence analysis primers. Freeman, New York, pp 1–59

  • Sambrook, Fritsch, Maniatis (1989) Molecular cloning, a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.

  • Sanangelantoni AM, Calogero RC, Buttarelli FR, Gualerzi CO, Tiboni O (1990) Organization and nucleotide sequence of the genes for ribosomal protein S2 and elongation factor Ts in Spirulina platensis. FEMS Microbiol Lett 54:141–145

    Article  CAS  PubMed  Google Scholar 

  • Serhir B, Dugourd D, Jacques M, Higgins R, Harel J (1997) Cloning and characterization of a dextranase gene (dexS) from Streptococcus suis. Gene 190:257–261

    CAS  PubMed  Google Scholar 

  • Seshadri R, Hendrix LR, Samuel JE (1999) Differential expression of translational elements by life cycle variants of Coxiella burnetii. Infect Immun 67:6026–6033

    CAS  PubMed  Google Scholar 

  • Wieden HJ, Wintermeyer W, Rodnina MV (2001) A common structural motif in elongation factor Ts and ribosomal protein L7/12 may be involved in the interaction with elongation factor Tu. J Mol Evol 52:129–136

    CAS  PubMed  Google Scholar 

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Acknowledgements

This study was supported by the Canadian Network on Bacterial Pathogens of Swine, grant number 225155-00.

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Authors and Affiliations

  1. Groupe de Recherche sur les Maladies Infectieuses du Porc and Canadian Network on Bacterial Pathogens of Swine, Faculté de médecine vétérinaire, Université de Montréal, C.P. 5000, St-Hyacinthe, Québec, J2S 7C6, Canada

    Gabriela Martinez, Josée Harel, Sonia Lacouture & Marcelo Gottschalk

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  1. Gabriela Martinez
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  2. Josée Harel
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  3. Sonia Lacouture
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  4. Marcelo Gottschalk
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Correspondence to Marcelo Gottschalk.

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Martinez, G., Harel, J., Lacouture, S. et al. Cloning and partial characterization of the gene encoding the putative elongation factor Ts of Streptococcus suis serotype 2. Arch Microbiol 180, 298–302 (2003). https://doi.org/10.1007/s00203-003-0595-7

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  • DOI: https://doi.org/10.1007/s00203-003-0595-7

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