Abstract.
The nucleotide sequence of a 6863-bp Spiroplasma citri DNA fragment comprising the spiralin gene was determined. Sequence analysis revealed eight putative ORFs that encode ribosomal protein S2, elongation factor Ts, spiralin, 6-phosphofructokinase, pyruvate kinase, and three unidentified proteins (A, B, and X). The gene organization reported here is different from that previously published. Northern blot analysis of rpsB, tsf, and x transcripts indicates that these genes are organized into a single transcriptional unit (operon). However, the detection of an additional transcript corresponding to the rpsB gene alone suggests that a transcriptional mechanism should occur in the 3′ region of the rpsB gene, allowing a conditional transcription termination.
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Received: 25 February 1998 / Accepted: 12 May 1998
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Le Dantec, L., Bové, J. & Saillard, C. Gene Organization and Transcriptional Analysis of the Spiroplasma citri rpsB/tsf/x Operon. Curr Microbiol 37, 269–273 (1998). https://doi.org/10.1007/s002849900377
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DOI: https://doi.org/10.1007/s002849900377