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The putrescine analogue 1-aminooxy-3-aminopropane perturbs polyamine metabolism in the phytopathogenic fungus Sclerotinia sclerotiorum

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Abstract

The effects of the putrescine analogue 1-aminooxy-3-aminopropane on fungal polyamine metabolism were evaluated using Sclerotinia sclerotiorum as an experimental model. The compound inhibited ornithine decarboxylase, spermidine synthase, and S -adenosyl-methionine decarboxylase in mycelial extracts. Addition of 1-aminooxy-3-aminopropane at 1 mM to the culture medium did not reduce mycelial growth and caused a 29% decrease in free spermidine and a two-fold increase in free spermine. When added 4.5 h before the determination of ornithine decarboxylase, 1-aminooxy-3-aminopropane reduced in vivo activity of this enzyme by 40–50%. When added 48 h before the determination, 1-aminooxy-3-aminopropane at 0.01 and 0.1 mM caused a slight increase of in vivo ornithine decarboxylase activity, while it had no effect at 1 mM. Comparison of the action of 1-aminooxy-3-aminopropane with that of other inhibitors of polyamine biosynthesis suggested that its effects on in vivo ornithine decarboxylase activity resulted from a balance between direct inhibition of enzyme activity and indirect stimulation of enzyme synthesis and/or activity mediated by the decrease in spermidine levels, which in turn was due to inhibition of spermidine synthase and S -adenosyl-methionine decarboxylase. The potential of 1-aminooxy-3-aminopropane as a tool for studies on fungal polyamine metabolism and for the control of plant diseases of fungal origin is discussed.

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Fig. 1A–E.
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Abbreviations

AdoMetDC :

S-Adenosyl-methionine decarboxylase

DFMO :

α-Difluoromethylornithine

MGBG :

Methylglyoxal bis-[guanyl hydrazone]

ODC :

Ornithine decarboxylase

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Acknowledgements

This work was supported by grants from the Third World Academy of Sciences (TWAS) and Agencia Española de Cooperación Internacional (AECI). AG, MCD and FLP are fellows of Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC), Universidad Nacional de General San Martín (UNSAM) and Fundación Consejo Regional de la Producción (COREPRO) respectively. OAR is a member of the research career from CONICET. We wish to thank Drs. A.F. Tiburcio and T. Altabella (University of Barcelona, Spain) for valuable comments and advice.

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  1. Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de General San Martín-Consejo Nacional de Investigaciones Científicas y Técnicas (UNSAM-CONICET), Casilla de Correo 164, 7130 , Chascomús, Buenos Aires, Argentina

    Andrés Gárriz, María C. Dalmasso, Fernando L. Pieckenstain & Oscar A. Ruiz

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  1. Andrés Gárriz
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  2. María C. Dalmasso
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  3. Fernando L. Pieckenstain
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  4. Oscar A. Ruiz
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Correspondence to Oscar A. Ruiz.

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Gárriz, A., Dalmasso, M.C., Pieckenstain, F.L. et al. The putrescine analogue 1-aminooxy-3-aminopropane perturbs polyamine metabolism in the phytopathogenic fungus Sclerotinia sclerotiorum . Arch Microbiol 180, 169–175 (2003). https://doi.org/10.1007/s00203-003-0572-1

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  • DOI: https://doi.org/10.1007/s00203-003-0572-1

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