Abstract
Summary
After a single cholecalciferol load, peak serum 25-hydroxycholecalciferol (25OHD) is lower in individuals with a higher body mass index (BMI), probably due to it being distributed in a greater volume. Its subsequent disappearance from the serum is slower the higher the individual’s BMI, probably due to the combination of a larger body volume and a slower release into the circulation of vitamin D stored in adipose tissue.
Introduction
The aim of the study is to examine 25-hydroxycholecalciferol (25OHD) response to a single oral load of cholecalciferol in the normal weight, overweight, and obese.
Methods
We considered 55 healthy women aged from 25 to 67 years (mean ± SD, 50.8 ± 9.5) with a BMI ranging from 18.7 to 42 kg/m2 (mean ± SD, 27.1 ± 6.0). The sample was divided into three groups by BMI: 20 were normal weight (BMI ≤ 25 kg/m2), 21 overweight (25.1 ≤ BMI ≤ 29.9 kg/ m2), and 14 obese (BMI ≥ 30 kg/m2). Each subject was given 300,000 IU of cholecalciferol orally during lunch. A fasting blood test was obtained before cholecalciferol loading and then 7, 30, and 90 days afterwards to measure serum 25OHD, 1,25 dihydroxyvitamin D [1,25 (OH)2D], parathyroid hormone (PTH), calcium (Ca), and phosphorus (P). Participants’ absolute fat mass was measured using dual energy X-ray absorptiometry (DEXA).
Results
The fat mass of the normal weight subjects was significantly lower than that of the overweight, which in turn was lower than that of the obese participants. Serum 25OHD levels increased significantly in all groups, peaking 1 week after the cholecalciferol load. Peak serum 25OHD levels were lower the higher the individuals’ BMI. After peaking, the 25OHD levels gradually decreased, following a significantly different trend in the three groups. The slope was similar for the overweight and obese, declining significantly more slowly than in the normal weight group. In the sample as a whole, there was a weakly significant negative correlation between fat mass and baseline 25OHD level, while this correlation became strongly significant at all time points after cholecalciferol loading.
Conclusions
The lower peak 25OHD levels seen in the obese and overweight is probably due to the cholecalciferol load being distributed in a larger body volume. The longer persistence of 25OHD in their serum could be due to both their larger body volume and a slower release into the circulation of the vitamin D stored in their adipose tissue.
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References
Höck AD (2014) Vitamin D3 deficiency results in dysfunctions of immunity with severe fatigue and depression in a variety of diseases. In Vivo 28(1):133–145
Lucas RM, Gorman S, Geldenhuys S, Hart PH (2014) Vitamin D and immunity. F1000 Prime Rep. 1;6:118
Zittermann A (2014) Vitamin D and cardiovascular disease. Anticancer Res 34(9):4641–4648
Romagnoli E, Pepe J, Piemonte S, Cipriani C, Minisola S (2013) Management of endocrine disease: value and limitations of assessing vitamin D nutritional status and advised levels of vitamin D supplementation. Eur J Endocrinol 169(4):R59–R69
Muscogiuri G, Mitri J, Mathieu C, Badenhoop K, Tamer G, Orio F, Mezza T, Vieth R, Colao A, Pittas A (2014) Mechanisms in endocrinology: vitamin D as a potential contributor in endocrine health and disease. Eur J Endocrinol 171(3):R101–R110
Shui I, Giovannucci E (2014) Vitamin D status and cancer incidence and mortality. Adv Exp Med Biol 810:33–51
Ordóñez Mena JM, Brenner H (2014) Vitamin D and cancer: an overview on epidemiological studies. Adv Exp Med Biol 810:17–32
Sai AJ, Walters RW, Fang X, Gallagher JC (2011) Relationship between vitamin D, parathyroid hormone, and bone health. J Clin Endocrinol Metab 96(3):E436–E446
Gandini S, Boniol M, Haukka J, Byrnes G, Cox B, Sneyd MJ, Mullie P, Autier P (2011) Meta-analysis of observational studies of serum 25-hydroxyvitamin D levels and colorectal, breast and prostate cancer and colorectal adenoma. Int J Cancer 128(6):1414–1424
Peterson CA, Tosh AK, Belenchia AM (2014) Vitamin D insufficiency and insulin resistance in obese adolescents. Ther Adv Endocrinol Metab 5(6):166–189
Cipriani C, Pepe J, Piemonte S, Colangelo L, Cilli M, Minisola S (2014) Vitamin D and its relationship with obesity and muscle. Int J Endocrinol 2014:841248. doi:10.1155/2014/841248
Gulseth HL, Gjelstad IM, Birkeland KI, Drevon CA (2013) Vitamin D and the metabolic syndrome. Curr Vasc Pharmacol 11(6):968–984
Vanlint S (2013) Vitamin D and obesity. Nutrients 5(3):949–956
Blum M, Dallal GE, Dawson-Hughes B (2008) Body size and serum 25 hydroxy vitamin D response to oral supplements in healthy older adults. J Am Coll Nutr 27(2):274–279
Lee P, Greenfield JR, Seibel MJ, Eisman JA, Center JR (2009) Adequacy of vitamin D replacement in severe deficiency is dependent on body mass index. Am J Med 122(11):1056–1060
Tepper S, Shahar DR, Geva D, Ish-Shalom S (2014) Predictors of serum 25(OH)D increase following bimonthly supplementation with 100,000 IU vitamin D in healthy, men aged 25-65 years. J Steroid Biochem Mol Biol 144 Pt A:163–166
Ekwaru JP, Zwicker JD, Holick MF, Giovannucci E, Veugelers PJ (2014) The importance of body weight for the dose response relationship of oral vitamin D supplementation and serum 25-hydroxyvitamin D in healthy volunteers. PLoS One 9(11):e111265
Montomoli M, Gonnelli S, Giacchi M, Mattei R, Cuda C, Rossi S, Gennari C (2002) Validation of a food frequency questionnaire for nutritional calcium intake assessment in Italian women. Eur J Clin Nutr 56(1):21–30
Glanz K, Yaroch AL, Dancel M, Saraiya M, Crane LA, Buller DB, Manne S, O’Riordan DL, Heckman CJ, Hay J, Robinson JK (2008) Measures of sun exposure and sun protection practices for behavioral and epidemiologic research. Arch Dermatol 144(2):217–222
Società Italiana dell’Osteoporosi, del Metabolismo Minerale e delle Malattie dello Scheletro (2013) Guidelines for the diagnosis, prevention and treatment of osteoporosis. Italian Osteoporosis, Mineral Metabolism, and Skeletal Diseases Society. Minerva Endocrinol 38(1 Suppl 1):1–30
Saneei P, Salehi-Abargouei A, Esmaillzadeh A (2013) Serum 25-hydroxyvitamin D levels in relation to body mass index: a systematic review and meta-analysis. Obes Rev 14(5):393–404
Holick MF (2004) Sunlight and vitamin D for bone health and prevention of autoimmune diseases, cancers, and cardiovascular disease. Am J Clin Nutr 80(6 Suppl):1678S–1688S
Foo LH, Teo PS, Abdullah NF, Aziz ME, Hills AP (2013) Relationship between anthropometric and dual energy X-ray absorptiometry measures to assess total and regional adiposity in Malaysian adolescents. Asia Pac J Clin Nutr 22(3):348–356
Drincic AT, Armas LA, Van Diest EE, Heaney RP (2012) Volumetric dilution, rather than sequestration best explains the low vitamin D status of obesity. Obesity 20(7):1444–1448
Drincic A, Fuller E, Heaney RP, Armas LA (2013) 25-Hydroxyvitamin D response to graded vitamin D3 supplementation among obese adults. J Clin Endocrinol Metab 98(12):4845–4851
Didriksen A, Burild A, Jakobsen J, Fuskevåg OM, Jorde R (2015) Vitamin D3 increases in abdominal subcutaneous fat tissue after supplementation with vitamin D3. Eur J Endocrinol 172(3):235–241
Brock K, Cant R, Clemson L, Mason RS, Fraser DR (2007) Effects of diet and exercise on plasma vitamin D (25OHD) levels in Vietnamese immigrant elderly in Sydney, Australia. J Steroid Biochem Mol Biol 103(3-5):786–792
Abboud M, Puglisi DA, Davies BN, Rybchyn M, Whitehead NP, Brock KE, Cole L, Gordon-Thomson C, Fraser DR, Mason RS (2013) Evidence for a specific uptake and retention mechanism for 25-hydroxyvitamin D (25OHD) in skeletal muscle cells. Endocrinology 154(9):3022–3030
Rowling MJ, Kemmis CM, Taffany DA, Welsh J (2006) Megalin-mediated endocytosis of vitamin D binding protein correlates with 25-hydroxycholecalciferol actions in human mammary cells. J Nutr 136(11):2754–2759
Abboud M, Gordon-Thomson C, Hoy AJ, Balaban S, Rybchyn MS, Cole L, Su Y, Brennan-Speranza TC, Fraser DR, Mason RS (2014) Uptake of 25-hydroxyvitamin D by muscle and fat cells. J Steroid Biochem Mol Biol 144 Pt A:232–236
Haddad JG, Matsuoka LY, Hollis BW, Hu YZ, Wortsman J (1993) Human plasma transport of vitamin D after its endogenous synthesis. J Clin Invest 91(6):2552–2555
Jones G (2008) Pharmacokinetics of vitamin D toxicity. Am J Clin Nutr 88(2):582S–586S
Piccolo BD, Dolnikowski G, Seyoum E, Thomas AP, Gertz ER, Souza EC, Woodhouse LR, Newman JW, Keim NL, Adams SH, Van Loan MD (2013) Association between subcutaneous white adipose tissue and serum 25-hydroxyvitamin D in overweight and obese adults. Nutrients 5(9):3352–3366
Mason C, Xiao L, Imayama I, Duggan CR, Bain C, Foster-Schubert KE, Kong A, Campbell KL, Wang CY, Neuhouser ML, Jeffery RW, Robien K, Alfano CM, Blackburn GL, McTiernan A (2011) Effects of weight loss on serum vitamin D in postmenopausal women. Am J Clin Nutr 94(1):95–103
Ritter CS, Armbrecht HJ, Slatopolsky E, Brown AJ (2011) 25-Hydroxyvitamin D(3) suppresses PTH synthesis and secretion by bovine parathyroid cells. Kidney Int 70(4):654–659
Ritter CS, Brown AJ (2011) Direct suppression of PTH gene expression by the vitamin D prohormones doxercalciferol and calcidiol requires the vitamin D receptor. J Mol Endocrinol 46(2):63–66
Grethen E, McClintock R, Gupta CE, Jones R, Cacucci BM, Diaz D, Fulford AD, Perkins SM, Considine RV, Peacock M (2011) Vitamin D and hyperparathyroidism in obesity. J Clin Endocrinol Metab 96(5):1320–1326
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Valentina Camozzi, Francesca Sanguin, Anna Chiara Frigo, Martina Zaninotto, Mario Plebani, Marco Boscaro, Lisa Schiavon, and Giovanni Luisetto have no conflict of interest to disclose.
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Camozzi, V., Frigo, A.C., Zaninotto, M. et al. 25-Hydroxycholecalciferol response to single oral cholecalciferol loading in the normal weight, overweight, and obese. Osteoporos Int 27, 2593–2602 (2016). https://doi.org/10.1007/s00198-016-3574-y
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DOI: https://doi.org/10.1007/s00198-016-3574-y