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Validation of a simple isotope method for estimating true calcium fractional absorption in adolescents

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Abstract

Summary

We validated a single oral isotope method for estimating fractional calcium absorption determined by double isotope methods in adolescents. Developed equations with an oral isotope including a single blood draw or spot urine collection can be used to evaluate fractional calcium absorption in adolescents which allows flexibility in developing protocols.

Introduction

This study was designed to develop and validate a simpler, less expensive single oral isotope method for determining fractional calcium (Ca) absorption in adolescents.

Methods

We used our database of 31 observations from ten white and 12 black adolescent girls aged 10-15 years who participated in metabolic and kinetic studies. Tracer data following oral (44Ca) and intravenous (IV, 42Ca) administration of calcium stable isotopes and samples in serum and urine from various time points up to 4 days were used to develop methods using multiple regression analysis based on a single measurement of enriched stable isotope/tracee defined as tracer/tracee (TT) in serum (TTserum) or urine (TTurine). Reference values for fractional calcium absorption were from oral/IV stable isotope ratios in 24-h serum or urine and full kinetic modeling.

Results

The strongest equation using a single blood sample had R 2 = 0.94 (p < 0.001): fractional Ca absorption = 1.3340(4-h TTserum)0.7872 BSA1.7132e (−0.01652 PMA), where BSA is body surface area and PMA is post-menarcheal age. The strongest equation using a single urine sample had R 2 = 0.95 (p < 0.001): fractional Ca absorption = 2.3088 (5-12 h TTurine)0.8208 BSA1.5260e (−0.01850 PMA). Equations were also developed with Tanner score. An external data set of Asian adolescent boys and girls was used to validate the equations.

Conclusion

Equations using an oral isotope and a single blood draw or urine collection for determining fractional calcium absorption were successfully validated in healthy, non-obese white and black adolescent girls aged 10-15 years. The equations well-predicted fractional calcium absorption in Asian adolescent boys and girls.

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Acknowledgment

We are grateful for the insights provided by Robert Heaney that helped to guide the preparation of this manuscript.

Author Lee was supported by NIH grant P50-AT00477.

Conflicts of interest

None.

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Authors and Affiliations

  1. Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA

    W. Lee

  2. Statistics, Purdue University, West Lafayette, IN, USA

    G. P. McCabe

  3. Department Foods and Nutrition, Purdue University, 700 West State St., West Lafayette, IN, 47907-2059, USA

    B. R. Martin & C. M. Weaver

Authors
  1. W. Lee
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  2. G. P. McCabe
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  3. B. R. Martin
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  4. C. M. Weaver
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Corresponding author

Correspondence to C. M. Weaver.

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Lee, W., McCabe, G.P., Martin, B.R. et al. Validation of a simple isotope method for estimating true calcium fractional absorption in adolescents. Osteoporos Int 22, 159–166 (2011). https://doi.org/10.1007/s00198-010-1203-8

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  • DOI: https://doi.org/10.1007/s00198-010-1203-8

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