Abstract
This paper proposes an automatic method for cell segmentation and classification of erythrocytes in thin blood smears with hemolytic anemia. First, to remove the background and noises in the blood images, the proposed method detects a series of changes on the edges and analyzes the edge changes by using the 8-connection chain codes technique to recognize isolated erythrocytes. For segmenting the overlapping erythrocytes, the 8-connection chain codes technique obtains the edge direction of the cells to effectively figure out the points of high concavity. Then, the adapted high concavity information is used to separate overlapping erythrocytes and to extract features from each segmented erythrocyte. After segmenting, all the erythrocytes can be treated equally and the differences between adjacent chain codes of each erythrocyte can be calculated. Furthermore, the proposed method extracts the variation of eight directions from each individual erythrocyte as their features for classifying into four main hemolytic anemia types. Finally, classification process identifies abnormal erythrocytes and the types of hemolytic anemia by using a trained bank of classifiers, utilizing the proposed method to calculate the quantity of erythrocytes and recognize the types of hemolytic anemia effectively.
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Doi, K.: Computer-aided diagnosis in medical imaging: historical review, current status and future potential. Comput. Med. Imaging Gr. Off. J. Comput. Med. Imaging Soc. 31(4–5), 198 (2007)
Shiraishi, J., Li, Q., Appelbaum, D., Doi, K.: Computer-aided diagnosis and artificial intelligence in clinical imaging. Semin. Nucl. Med. 41(6), 449–462 (2011)
Sharma, N., Aggarwal, L.M.: Automated medical image segmentation techniques. J Med. Phys./Assoc. Med. Phys. India 35(1), 3 (2010)
Clas, P., Groeschel, S., Wilke, M.: A semi-automatic algorithm for determining the demyelination load in metachromatic leukodystrophy. Acad. Radiol. 19(1), 26–34 (2012)
Adollah, R., Mashor, M.Y., Mohd Nasir, N.F., Rosline, H., Mahsin, H., Adilah, H.: Blood cell image segmentation: a review. In: 4th Kuala Lumpur International Conference on Biomedical Engineering (2008)
Ballarò, B., Florena, A.M., Franco, V., Tegolo, D., Tripodo, C., Valenti, C.: An automated image analysis methodology for classifying megakaryocytes in chronic myeloproliferative disorders. Med. Image Anal. 12(6), 703–712 (2008)
Shapiro, Linda G., Stockman, George C.: Computer Vision. Prentice-Hall, New Jersey (2001)
Pham, D.L., Xu, C., Prince, J.L.: Current methods in medical image segmentation. Annu. Rev. Biomed. Eng. 2(1), 315–337 (2000)
Sapna Varshney, S., Rajpal, N., Purwar, R.: Comparative study of image segmentation techniques and object matching using segmentation. In: Methods and Models in Computer Science. ICM2CS (2009)
Levin, A., Zomet, A., Peleg, S., Weiss, Y.: Seamless Image Stitching in the Gradient Domain. Computer Vision-ECCV (2004)
Dorini, L.B., Minetto, R., Leite, N.J.: White blood cell segmentation using morphological operators and scale-space analysis. In: Computer Graphics and Image Processing. SIBGRAPI (2007)
Vizireanu, D.N., Pirnog, C., Lãzãrescu, V., Vizireanu, A.: The skeleton structure—an improved compression algorithm with perfect reconstruction. J Digit. Imaging 14(2), 241–242 (2001)
Vizireanu, D.N., Halunga, S., Fratu, O.: A grayscale image interpolation method using new morphological skeleton. In: Telecommunications in Modern Satellite, Cable and Broadcasting Service. TELSIKS (2003)
Mohamed, M., Far, B.: An enhanced threshold based technique for white blood cells nuclei automatic segmentation. In: IEEE 14th International Conference on e-Health Networking, Applications and Services (Healthcom) (2012)
Hiremath, P.S., Bannigidad, P., Geeta, S.: Automated identification and classification of white blood cells (leukocytes) in digital microscopic images. IJCA Special Issue on “Recent Trends in Image Processing and Pattern Recognition” RTIPPR (2010)
Fatichah, C., Tangel, M.L., Widyanto, M.R., Dong, F., Hirota, K.: Parameter optimization of local fuzzy patterns based on fuzzy contrast measure for white blood cell texture feature extraction. J. Ref. J. Adv. Comput. Intell. Intell. Inform. 16(3), 412–419 (2012)
Sharif, J.M., Miswan, M.F., Ngadi, M.A., Salam, M.S.H., Mahadi bin Abdul Jamil, M.: Red blood cell segmentation using masking and watershed algorithm: a preliminary study. In: International Conference on Biomedical Engineering (ICoBE) (2012)
Khan, A.M., El-Daly, H., Rajpoot, N.M.: A Gamma–Gaussian mixture model for detection of mitotic cells in breast cancer histopathology images. In: 21st International Conference on Pattern Recognition (ICPR) (2012)
Hahn, K., Jung, S., Han, Y., Hahn, H.: A new algorithm for ellipse detection by curve segments. Pattern Recognit. Lett. 29(13), 1836–1841 (2008)
Jung, C., Kim, C., Chae, S.W., Oh, S.: Unsupervised segmentation of overlapped nuclei using Bayesian classification. IEEE Trans. Biomed. Eng 57(12), 2825–2832 (2010)
Freeman, H.: Computer processing of line-drawing images. ACM Comput. Surv. 6(1), 57–97 (1974)
Sánchez-Cruz, H., Bribiesca, E., Rodríguez-Dagnino, R.M.: Efficiency of chain codes to represent binary objects. Pattern Recognit. 40(6), 1660–1674 (2007)
Huang, D.Y., Wang, C.H.: Optimal multi-level thresholding using a two-stage Otsu optimization approach. Pattern Recognit. Lett. 30(3), 275–284 (2009)
Rodríguez, R.: A strategy for blood vessels segmentation based on the threshold which combines statistical and scale space filter: application to the study of angiogenesis. Comput. Methods Programs Biomed. 82(1), 1–9 (2006)
Yu, D., Pham, T.D., Zhou, X., Wong, S.T.: Recognition and analysis of cell nuclear phases for high-content screening based on morphological features. Pattern Recognit. 42(4), 498–508 (2009)
Theera-Umpon, N., Dhompongsa, S.: Morphological granulometric features of nucleus in automatic bone marrow white blood cell classification. IEEE Trans. Inf. Technol. Biomed. 11(3), 353–359 (2007)
Weka 3: Data Mining Software in Java. http://www.cs.waikato.ac.nz/ml/weka/ (2013)
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Chen, HM., Tsao, YT. & Tsai, SN. Automatic image segmentation and classification based on direction texton technique for hemolytic anemia in thin blood smears. Machine Vision and Applications 25, 501–510 (2014). https://doi.org/10.1007/s00138-013-0585-y
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DOI: https://doi.org/10.1007/s00138-013-0585-y