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Mutagenicity and Genotoxicity in Gill Erythrocyte Cells of Poecilia reticulata Exposed to a Glyphosate Formulation

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Abstract

Poecilia reticulata were exposed to herbicide Roundup Transorb® for micronucleus test, nuclear abnormalities and comet assay. The exposure-concentrations were based on CL50–96 h following 0, 1.41, 2.83, 4.24 and 5.65 μL L−1 for 24 h. Micronucleus and comets were significantly increased in the gill erythrocyte cells after herbicide exposure compared with the non-exposed group. Results showed a gradual increase in the number of damaged cells, indicating a concentration-dependent effect and that this herbicide was mutagenic and genotoxic to P. reticulata and this effect could be attributed to a combination of compounds contained in the formulation with the active ingredient glyphosate.

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Acknowledgments

This study was supported by the National Council of Scientific and Technologic Development (CNPq-Brazil). J. de Souza Filho had a fellowship from CNPq.

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Authors and Affiliations

  1. Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, CEP 70919-970, Brazil

    José De Souza Filho & Cesar Koppe Grisolia

  2. Departamento de Biologia, Universidade Católica de Goiás, Goiânia, Brazil

    Caio César Neves Sousa & Cláudio Carlos Da Silva

  3. Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Brazil

    Simone Maria Teixeira De Sabóia-Morais

Authors
  1. José De Souza Filho
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  2. Caio César Neves Sousa
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  3. Cláudio Carlos Da Silva
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  4. Simone Maria Teixeira De Sabóia-Morais
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  5. Cesar Koppe Grisolia
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Correspondence to Cesar Koppe Grisolia.

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De Souza Filho, J., Sousa, C.C.N., Da Silva, C.C. et al. Mutagenicity and Genotoxicity in Gill Erythrocyte Cells of Poecilia reticulata Exposed to a Glyphosate Formulation. Bull Environ Contam Toxicol 91, 583–587 (2013). https://doi.org/10.1007/s00128-013-1103-7

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  • DOI: https://doi.org/10.1007/s00128-013-1103-7

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