Mutagenicity and Genotoxicity in Gill Erythrocyte Cells of Poecilia reticulata Exposed to a Glyphosate Formulation
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Poecilia reticulata were exposed to herbicide Roundup Transorb® for micronucleus test, nuclear abnormalities and comet assay. The exposure-concentrations were based on CL50–96 h following 0, 1.41, 2.83, 4.24 and 5.65 μL L−1 for 24 h. Micronucleus and comets were significantly increased in the gill erythrocyte cells after herbicide exposure compared with the non-exposed group. Results showed a gradual increase in the number of damaged cells, indicating a concentration-dependent effect and that this herbicide was mutagenic and genotoxic to P. reticulata and this effect could be attributed to a combination of compounds contained in the formulation with the active ingredient glyphosate.
KeywordsRoundup Transorb® Comet assay Micronucleus Glyphosate formulations
This study was supported by the National Council of Scientific and Technologic Development (CNPq-Brazil). J. de Souza Filho had a fellowship from CNPq.
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