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Molecular mapping of the rf1 gene for pollen fertility restoration in sorghum (Sorghum bicolor L.)

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Abstract 

We report the molecular mapping of a gene for pollen fertility in A1 (milo) type cytoplasm of sorghum using AFLP and SSR marker analysis. DNA from an F2 population comprised of 84 individuals was screened with AFLP genetic markers to detect polymorphic DNAs linked to fertility restoration. Fifteen AFLP markers were linked to fertility restoration from the initial screening with 49 unique AFLP primer combinations (+3/+3 selective bases). As many of these AFLP markers had been previously mapped to a high-density genetic map of sorghum, the target gene (rf1) could be mapped to linkage group H. Confirmation of the map location of rf1 was obtained by demonstrating that additional linkage group-H markers (SSR, STS, AFLP) were linked to fertility restoration. The closest marker, AFLP Xtxa2582, mapped within 2.4 cM of the target loci while two SSRs, Xtxp18 and Xtxp250, flanked the rf1 locus at 12 cM and 10.8 cM, respectively. The availability of molecular markers will facilitate the selection of pollen fertility restoration in sorghum inbred-line development and provide the foundation for map-based gene isolation.

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Authors and Affiliations

  1. USDA-ARS, Southern Plains Agricultural Research Center, College Station TX 77845, USA e-mail: Bob@algodon.tamu.edu Fax: (979)-260-9333, Tel.: (979)-777-4470, , , , , , US

    R. R. Klein & K. F. Schertz

  2. Department of Biochemistry-Biophysics and Institute for Plant Genomics and Biotechnology Texas A&M University, College Station, TX 77843, USA, , , , , , US

    P. E. Klein, K. L. Childs & J. E. Mullet

  3. Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX 77843, USA, , , , , , US

    A. K. Chhabra, J. Dong & S. Pammi

  4. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX 77843, USA, , , , , , US

    W. L. Rooney

Authors
  1. R. R. Klein
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  2. P. E. Klein
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  3. A. K. Chhabra
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  4. J. Dong
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  5. S. Pammi
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  6. K. L. Childs
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  7. J. E. Mullet
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  8. W. L. Rooney
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  9. K. F. Schertz
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Additional information

Received: 22 August 2000 / Accepted: 18 October 2000

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Klein, R., Klein, P., Chhabra, A. et al. Molecular mapping of the rf1 gene for pollen fertility restoration in sorghum (Sorghum bicolor L.). Theor Appl Genet 102, 1206–1212 (2001). https://doi.org/10.1007/s001220100575

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  • DOI: https://doi.org/10.1007/s001220100575

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