Abstract
The effect of additional virulence (vir) genes and size of plasmid T-DNA in Agrobacterium tumefa- ciens was investigated for their impact on transformation efficiency. Transformation efficiency in tobacco, cotton, and rice was increased when the T-DNA was 4.3 kb compared to 8.4 kb in size. However, when additional virG, virGN54D,virE, or virE/virG plasmids were included with the 8.4-kb T-DNA, transformation frequencies in all cases were increased over that of the shorter T-DNA without additional vir plasmids. The use of virE, virG or virGN54D copies enhanced transformation efficiency; however, the most significant increase of transformation efficiency in all three plant species was observed when the virE/virG plasmid was used for infection. The virE/virG plasmid dramatically enhanced the efficiency of Agrobacterium-mediated gene transfer; moreover, this plasmid appears to have broad efficiency since it was consistently effective on two different dicotyledon species as well as a monocotyledon species.
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Received: 8 February 2000 / Accepted: 21 March 2000
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Park, S., Lee, BM., Salas, M. et al. Shorter T-DNA or additional virulence genes improve Agrobactrium-mediated transformation. Theor Appl Genet 101, 1015–1020 (2000). https://doi.org/10.1007/s001220051575
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DOI: https://doi.org/10.1007/s001220051575