Skip to main content
SpringerLink
Log in

Locating the petunia Rf gene on a 650-kb DNA fragment

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

 A bulked segregant analysis was conducted in order to find RAPD and AFLP markers linked to the restorer of fertility (Rf ) gene in petunia. One RAPD marker, OP704, and one AFLP marker, ECCA/ MACT, were found to be closely linked to Rf (<1 cM) in our mapping population produced from an intraspecific Petunia hybrida cross. These two single-copy markers bracketing Rf were then mapped as RFLPs on the tomato map. Despite some rearrangement between the petunia and the tomato genomes, this synteny survey revealed two tomato markers, TG250 and CT24, closely linked to Rf. Physical mapping indicates that CT24, OP704 and ECCA/MACT lie on the same 650-kb MluI fragment. A physical to genetic distance ratio of 400 kb/cM around the Rf gene should make it feasible to identify markers physically very close to Rf.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Section of Genetics and Development, 321 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA Fax: +1 (607)-255-6249 E-mail: mrh5@cornell.edu, , , , , , US

    S. Bentolila & M. R. Hanson

  2. Laboratorium voor Genetica, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium, , , , , , BE

    J. Zethof & T. Gerats

Authors
  1. S. Bentolila
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Zethof
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Gerats
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. R. Hanson
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 20 August 1997 / Accepted: 21 October 1997

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bentolila, S., Zethof, J., Gerats, T. et al. Locating the petunia Rf gene on a 650-kb DNA fragment. Theor Appl Genet 96, 980–988 (1998). https://doi.org/10.1007/s001220050829

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s001220050829

Search

Navigation