Abstract
Key message
Two QTLs with major effects on rolled leaf trait were consistently detected on chromosomes 1A (QRl.hwwg-1AS) and 5A (QRl.hwwg-5AL) in the field experiments.
Abstract
Rolled leaf (RL) is a morphological strategy to protect plants from dehydration under stressed field conditions. Identification of quantitative trait loci (QTLs) underlining RL is essential to breed drought-tolerant wheat cultivars. A mapping population of 154 recombinant inbred lines was developed from the cross between JagMut1095, a mutant of Jagger, and Jagger to identify quantitative trait loci (QTLs) for the RL trait. A linkage map of 3106 cM was constructed with 1003 unique SNPs from 21 wheat chromosomes. Two consistent QTLs were identified for RL on chromosomes 1A (QRl.hwwg-1AS) and 5A (QRl.hwwg-5AL) in all field experiments. QRl.hwwg-1AS explained 24–56% of the phenotypic variation and QRl.hwwg-5AL explained up to 20% of the phenotypic variation. The combined percent phenotypic variation associated with the two QTLs was up to 61%. Analyses of phenotypic and genotypic data of recombinants generated from heterogeneous inbred families of JagMut1095 × Jagger delimited QRl.hwwg-1AS to a 6.04 Mb physical interval. This work lays solid foundation for further fine mapping and map-based cloning of QRl.hwwg-1AS.
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Acknowledgements
This is contribution number 23-030-J from the Kansas Agricultural Experiment Station. Author thanks Dr. Michael Pumphrey for providing the Jagger mutant population. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer.
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Funding is provided by the US Wheat and Barley Scab Initiative, the Agriculture and Food Research Initiative Competitive Grant 2022-68013-36439 (WheatCAP) from the USDA National Institute of Food and Agriculture, and Chinese Scholarship Council.
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GB designed the research; RB, YX, SAP, and AB performed the research. NL and ZS conducted mutant screening. EA and KJ provided exome-capture data. RB, GZ, AF, and JR performed field phenotyping. RB and GB wrote the manuscript. All the authors provided inputs and approved the manuscript.
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Bian, R., Liu, N., Xu, Y. et al. Quantitative trait loci for rolled leaf in a wheat EMS mutant from Jagger. Theor Appl Genet 136, 52 (2023). https://doi.org/10.1007/s00122-023-04284-3
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DOI: https://doi.org/10.1007/s00122-023-04284-3