Abstract
Key message
Identified a recessive gene (Cmpmr2F) associated with resistance to infection by the powdery mildew causing agent Podosphaera xanthii race 2F.
Abstract
Powdery mildew (PM) is one of the most destructive fungal diseases of melon, which significantly reduces the crop yield and quality. Multiple studies are being performed for in-depth genetic understandings of PM-susceptibility or -resistance mechanisms in melon plants, but the holistic knowledge of the precise genetic basis of PM-resistance is unexplored. In this study, we characterized the recessive gene “Cmpmr2F” and found its association with resistance against the PM causative agent “Podosphaera xanthii race 2F.” Fine genetic mapping revealed the major-effect region of a 26.25-kb interval on chromosome 12, which harbored the Cmpmr2F gene corresponding to the MELO3C002403, encoding allantoate amidohydrolase. The functional gene annotation, expression pattern, and sequence alignment analyses were carried out using two contrast parent lines of melon “X055” PM-susceptible and “PI 124112” PM-resistant. Further, gene silencing of Cmpmr2F using virus-induced gene silencing (VIGS) significantly increased PM-resistance in the susceptible plant. In contrast to the previously reported studies, we identified that Cmpmr2F-silenced plants showed no impairment in growth due to less apparent negative effects in silenced melon plants. So, it is believed that the Cmpmr2F gene has great potential for further breeding studies to increase the P. xanthii race 2F resistance in melon. In short, our study provides new genetic resources and a solid foundation for further functional analysis of PM-resistance genes in melon, as well as powerful molecular markers for marker-assisted breeding aimed at developing new melon varieties resistant to PM infection.
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Change history
23 March 2023
A Correction to this paper has been published: https://doi.org/10.1007/s00122-023-04323-z
07 April 2023
A Correction to this paper has been published: https://doi.org/10.1007/s00122-023-04324-y
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Acknowledgements
The authors are thankful to the Zhengzhou Fruit Research Institute of the Chinese Academy of Agricultural Sciences, for providing the VIGS vector. This work was supported by the National Nature Science Foundation of China (No. U21A20229).
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This work was supported by the National Nature Science Foundation of China (No. U21A20229).
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P.G. designed this research. T.F.Z. completed the experiments and wrote this article. H.N.C. performed the experimental data analysis. F.S.L. provided technical support for fine mapping. H.Y.L. developed the experimental populations of melon. S.A. performed the reviewing, writing, and editing of this manuscript. Z.D., M.L.Z., and T.T.M. participated in DNA extraction.
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Zhang, T., Cui, H., Luan, F. et al. A recessive gene Cmpmr2F confers powdery mildew resistance in melon (Cucumis melo L.). Theor Appl Genet 136, 4 (2023). https://doi.org/10.1007/s00122-023-04269-2
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DOI: https://doi.org/10.1007/s00122-023-04269-2